摘要
海带渣是海带加工过程中主要固体废弃物。本实验利用海带渣对生防菌株JGA2(-5)27-2进行固体发酵。实验对不同发酵条件下发酵产物对植物病原真菌寄生曲霉的抑制活性与对发酵产植物激素6-糠氨基嘌呤的能力进行测定。通过单因素实验和响应面实验优化了发酵条件:蔗糖为最优的碳源;硝酸盐为最优的氮源;蔗糖浓度、KNO3浓度、最佳的培养时间、含水量、培养温度、接种量和pH值分别为2.091%、0.507%、8.24 d、60%、35℃、4%和自然pH值。在发酵过程中以添加KNO3的形式向发酵菌肥中加入K+离子。实验证明,利用生防菌株JGA2(-5)27-2进行海带渣的固体发酵生产多功能微生物肥料具有可行性,并为海带渣的资源化在农业领域的利用提供一定参考。
Kelp residue is the main waste of kelp processing. Kelp residue solid fermentation inoculated with one Bacillus subtilis strains JGA2(-5) 27-2 was studied through this research. Inhibitory activity against Aspergillus parasiticus and the production of plant hormones 6-furfurylaminopurine under different fermentation conditions were measured. Single factor experiment and response surface methodology were done to optimize the fermentation conditions. Results showed that sucrose and nitrates were the optimal carbon source and the optimal nitrogen source,respectively. Optimal incubation sucrose concentration,potassium nitrate concentration,incubation time,moisture content,incubation temperature,inoculation amount,and pH were 2. 091%,0. 507%,8. 24 days,60%,35℃ and 4%,and natural,respectively. K+ion was introduced into microbial fertilizer in the form of KNO3 as plus nitrogen. The research not only shown that to produce microbial fertilizer by using kelp residue solid was also feasible,but also provided reference for kelp residue resource utilization in the field of agriculture.
出处
《环境工程学报》
CAS
CSCD
北大核心
2014年第11期4984-4990,共7页
Chinese Journal of Environmental Engineering
基金
威海市科技发展研究计划(2010-3-96)
哈尔滨工业大学优秀团队支持计划项目
关键词
海带渣
固体发酵
微生物肥料
资源化利用
kelp residue
solid fermentation
microbial fertilizer
resource utilization
