摘要
【目的】地膜覆盖会改变植烟土壤环境,进而影响土壤微生物群落结构和烤烟的生长发育,本研究旨在探究全生育期覆膜对植烟土壤理化性质和细菌群落结构的影响。【方法】应用16S rDNA测序,通过多种分析方法比较不同处理细菌群落结构的差异及多样性,并结合土壤理化性质进行冗余分析(RDA),以求明确土壤理化性质和细菌群落之间的关系。【结果】全生育期覆膜显著提高了烤烟产值量,双色(SY)、黑色(HY)、白色地膜覆盖(BY)处理产量分别较对照(CK)提高13.33%、18.24%、21.36%;产值较对照提高20.26%、15.87%、13.28%。同时,覆膜还会提高植烟土壤温度、总钾、总磷、总铁和有效铜含量,降低土壤pH值、碱解氮、有效铁、总铜和总锌含量。其中,全磷和有效铜含量增幅最为明显,分别为3.11%~27.33%和4.76%~19.05%,碱解氮、有效铁和全锌含量降幅最为显著,分别为10.45%~14.50%、3.36%~13.43%和7.69%~10.01%。全生育期覆膜对细菌群落丰度有一定抑制作用,对照的丰度指数Chao 1显著高于3个覆膜处理,且分别较白色、双色、黑色地膜覆盖处理高14.09%、24.20%、24.81%。冗余分析表明,影响细菌门水平群落结构的主要土壤理化因素是土壤温度、碱解氮、有效铁、总铁、有效铜和总铜含量。白色、双色地膜覆盖处理中存在3个共有与对照丰度明显不同的菌属,分别属于变形菌门(Proteobacteria)中的鞘脂单胞菌科(Sphingomonadaceae)、伯克霍尔德菌科(Burkholderaceae)和黄单胞菌科(Xanthomonadaceae)3个科。【结论】烤烟全生育期覆膜栽培会显著影响植烟土壤理化性质和细菌群落结构,提高烤烟产值量,适宜搭配的地膜颜色为黑白双色膜。
【Objective】The soil environment of tobacco planting can be impacted by plastic film mulching,which affects the structure of the soil microbial community and the growth and development of flue-cured tobacco.The purpose of this study is to investigate the effect of a full growth period mulching on the physicochemical properties and bacterial community structure of tobacco planting soil.【Method】16S rDNA sequencing was applied to compare the differences in the structure and diversity of bacterial communities in different treatments by various analytical methods.The connection between soil physicochemical properties and bacterial communities was discovered through redundancy analysis(RDA)based on soil physicochemical properties.【Result】The output value of flue-cured tobacco was significantly increased by film mulching during the growth period.The yield was increased by 13.33%,18.24%,and 21.36%by the double color film mulching(SY),black film mulching(HY),and white film mulching(BY)treatments,respectively,compared to the control(CK);Output value added was 20.26%,15.87%,and 13.28%.At the same time,film mulching will also increase tobacco-planting soil temperature,total potassium,total phosphorus,total iron and effective copper contents,and decrease soil pH value,alkaline dissolved nitrogen,effective iron,total copper and total zinc contents.The contents of total phosphorus and available copper increased the most significantly,reaching 3.11%-27.33%and 4.76%-19.05%,respectively.The most significant decrease in alkali hydrolyzed nitrogen,available iron,and total zinc occurred at 10.45%-14.50%,3.36%-13.43%,and 7.69%-10.01%,respectively.Plastic covering during the whole growth period has a certain negative impact on the abundance of bacterial communities.The abundance index Chao 1 of the control was significantly higher than the three mulching treatments and was 14.09%,24.20%,and 24.81%higher than that of the white film mulching,double color film mulching,and black film mulching treatments,respectively.Redundancy
作者
郭冲
曾淑华
刘雅洁
杨懿德
杨洋
鄢敏
陈佛源
韩伟
刘雷
GUO Chong;ZENG Shuhua;LIU Yajie;YANG Yide;YANG Yang;YAN Min;CHEN Foyuan;HAN Wei;LIU Lei(College of Agriculture,Sichuan Agricultural University,Chengdu 611130,China;Yibin Tobacco Company of Sichuan Province,Yibin 644600,China)
出处
《广东农业科学》
CAS
2023年第8期21-30,共10页
Guangdong Agricultural Sciences
基金
四川省烟草公司科技项目(2020511500200085,SCYC202306)。
关键词
全生育期覆膜
植烟土壤
理化性质
细菌群落结构
产值量
冗余分析
film mulching during the whole growth period
tobacco-planting soil
physicochemical properties
bacterial community structure
output value
redundancy analysis
