摘要
针对严重威胁烤烟大田生产的赤星病病害,培育抗病品种。以烤烟品种NC89为受体,具有选择标记性状的CV87为供体,采用浸种法将供体总DNA导入受体,获得具有目的性状的Do代变异株收种并种植后得到D1代,对其D1代进行实验检测结果表明浸种法直接导入外源DNA在D1代,仍可有效转移株高,株型,叶色,叶面积,生育期及抗病性等性状,并获得较高转化率D1代为15％。导入后D1代成熟烟叶总糖与蛋白质含量变异株与对照基本一致,说明变异株基本保持了受体原有的优良品质。蛋白质聚丙烯酰胺凝胶电泳检测,表明导入D1代变异株出现了受体没有的而供体所特有的两条谱带,Rr0.19和Rr0.63。具有供体特有的两条谱带的D1代变异株有A14B6.C5C10D3等5个优良株系。经过D1代一系列检测和筛选,选出5株早熟,优质,抗病变异株有A14.B6.C5.C10.D3。为今后进行D2代筛选,通过分子验证,为烟草育种创造新种质,开创新途径。
Aiming at the serous red star disease, a disease resistant tobacco clutivar was developed. Taking tobacco cultivar NC89 as receptor plants, cultivar CV87 as donor(with selective marker), the donor DNA was transformed through seed-immersion method. D1 generation which demonstrated the target characteristics was obtained from parent Do generation mutant. The determination of D1 generation indicated that:the phenotypes such as height, type, color leaves size, growth period and disease-resistance etc. were effectively transferred into D1 generation through seed-immersion DNA transformation, and the transformation rate was 15%.the total sugar and protein content of mature tobacco leaves were similar between the mutant and control in D1 generation, that is to say that the mutants still reserved the good characters of the receptor plants.The D, generation mutants exhibited two specific bands, named Rfo 19 and Rfo.w, that only belong to the donor plants, by SDS-PAGE analysis. The mutant cultivars were Au, Be, Cs, Cio, D].5 mutant cultivar with prematurity and disease-resistance characters were screened in Di generation, and it created a new way to develop new cultivar of tobacco.
出处
《农业与技术》
2002年第6期49-55,共7页
Agriculture and Technology
关键词
浸种法
外源DMA
转化
烤烟D1代
遗传性变异
Tobacco Red Star Disease Seed-Immersion method Transformation of Foreign DNA Mutation
