摘要
目的 建立甘芪胃疡宁颗料中芍药苷含量测定方法。方法 用HPLC测定 ,分析柱为ODS柱 (2 0 0mm×4 6mm ,5 μm) ,流动相为 0 0 5 %磷酸二氢钾溶液 -乙晴 (85∶15 ) ,检测波长 2 30nm ,流速为 0 9ml·min-1。结果 线性范围 0 2 31～ 1 15 8μg(r=0 9999)。平均回收率 98 2 7% ,RSD =0 86 % (n =6 )。 结论 该法简便、灵敏、准确。
OBJECTIVE To establish the method of determination paeoniflorin contents in Ganqi Weiyangning granules. METHODS HPLC was used.The HPLC system consisted of Diamonsil ODS column(200 mm×4.6 mm,5 μm),0.05%KH 2PO 4-acetonitrile(85∶15) as mobile phase. The flow rate was 0.9 ml·min -1 and the detection wavelength was 230 nm.RESULTS This method was simple,sensitive and accurate.The linear range was from 0.231ug to 1.158 μg( r =0.9999).The mean recovery was 98 27%, RSD of 0.86%.CONCLUSION The established method can be used for quality control of Ganqi Weiyangning granules.
出处
《华西药学杂志》
CAS
CSCD
2002年第6期457-458,共2页
West China Journal of Pharmaceutical Sciences
