摘要
分别采用Folin-Ciocalteu法测定了水果提取物的总酚含量,采用亚甲基蓝法测定了其抗人肝癌细胞Hep G2和人结肠腺癌细胞Caco-2增殖的活性,分析了总酚含量与抗Hep G2和Caco-2细胞增殖活性之间的相关性。结果显示,25种水果中李子的总酚含量最高(1686.08±96.94μmol GAE/100鲜果),西瓜(83.54±2.10μmol GAE/100 g鲜果)和哈密瓜(79.35±0.76μmol GAE/100 g鲜果)的总酚含量最低;在可测出抗增殖EC50值的水果中,李子(18.99±0.06 mg/m L)和番石榴(20.94±0.09 mg/m L)抗Hep G2细胞增殖的活性最强,梨的活性最弱(389.63±10.82 mg/m L)。李子抗Caco-2细胞增殖的活性最强(8.73±0.11 mg/m L),火龙果的活性最弱(388.07±7.04 mg/m L)。水果的抗Hep G2和Caco-2细胞增殖活性与其总酚含量相关性显著(R2=0.4147,p<0.01;R2=0.4071,p<0.05),该相关性表明水果中的多酚具有良好的抗肿瘤细胞增值的活性。
The total phenolic content and the anti-proliferative activities of the phenolic extracts against Hep G2 and Caco-2 cells were determined by the Folin-Ciocalteu method and the methylene blue assay, respectively; the correlation between the total phenolic content and the anti-proliferative activity of the extracts was analyzed. The results of these analyses revealed that plum(Prunus salicina Lindl.) had the highest total phenolic content(1686.08 ± 96.94 μmol gallic acid equivalent(GAE)/100 g of fresh fruit), whereas watermelon(Citrullus lanatus)(83.54 ± 2.10 μmol GAE/100 g of fresh fruit) and hami melon(Cucumis melo var. saccharinus)(79.35 ± 0.76 mol GAE/100 g of fresh fruit) had the lowest phenolic content among the 25 tested species. Among the fruit species with quantifiable anti-proliferative activities(half maximal effective concentration(EC50)), plum and guava(Psidium guajava Linn.) showed the strongest anti-proliferative activities against Hep G2 cells(EC50 values: 18.99 ± 0.06 and 20.94 ± 0.09 mg/m L, respectively), whereas pears(Pyrus spp.) showed the weakest anti-proliferative activity against these cells(EC50 value: 389.63 ± 10.82 mg/m L). Plum also demonstrated the strongest anti-proliferative activity against Caco-2 cells(EC50 value: 8.73 ± 0.11 mg/m L), while the activity of pitava(Hylocereus undulatus Britt.) was observed to be the weakest(EC50 value: 388.07 ± 7.04 mg/m L). The anti-proliferative activities of fruits on Hep G2 and Caco-2 cells were significantly correlated to their total phenolic content(R2 = 0.4147, p < 0.01; R2 = 0.4071, p < 0.05, respectively). This correlation indicates the high anti-proliferative effect of fruit polyphenols on cancer cells.
出处
《现代食品科技》
EI
CAS
北大核心
2015年第4期23-28,共6页
Modern Food Science and Technology
基金
广东省自然科学基金资助项目(S2011010004455)
深圳市科技计划项目(JC201005280530A
JCYJ20130331151222011)
广东省高等职业院校珠江学者岗位计划资助项目(2011)
关键词
水果
多酚
人结肠腺癌Caco-2细胞
人肝癌HEPG2细胞
抗增殖活性
fruit
polyphenol
HepG2 human hepatic carcinoma cell line
Caco-2 human colon adenocarcinoma cell line
anti-proliferative activity
