摘要
目的考察保加利亚杆菌、嗜热链球菌、嗜酸乳杆菌等几种常用乳制品益生菌对Caco-2细胞(结肠癌细胞)增殖的影响及其联合毒性。方法活化并培养保加利亚杆菌、嗜热链球菌和嗜酸乳杆菌,分别以不同浓度的益生菌处理培养好的Caco-2细胞,用MTT比色法测定益生菌对Caco-2细胞增殖的影响,并确定3种益生菌的半数抑制浓度(IC50)。将处于同一浓度级别的3种益生菌等比混合,进行联合毒性实验,用MTT法观察它们的联合毒性作用。结果 3种益生菌在低浓度(102、103、104 CFU/ml)未显示出细胞毒性,高浓度(105、106、107、108、109 CFI/ml)显示出显著的细胞毒性作用,细胞毒性作用随着浓度增加而增强,3种益生菌的IC50分别为:保加利亚杆菌IC50=1.90×108,嗜热链球菌IC50=1.86×109,嗜酸乳杆菌IC50=1.81×109。3种益生菌对Caco-2细胞的联合毒性作用与单一益生菌的细胞毒性作用差异无统计学意义(P>0.05),经计算为独立作用。结论保加利亚杆菌、嗜热链球菌和嗜酸乳杆菌对Caco-2细胞有细胞毒性作用,且呈剂量依赖性,但3种益生菌对Caco-2细胞没有联合毒性作用。
[Objective]To investigate the cytotoxicity and combined toxicity of several common probiotics in dairy products（Bulgarian bacillus,Streptococcus thermophilus,Lactobacillus acidophilus）on Caco-2 cells（colon cancer）. [Methods]Activating and culturing of Bulgaria bacillus,Streptococcus thermophilus and Lactobacillus acidophilus,then were cultured with Caco-2 cells.The effect and half inhibitory concentration（IC50） of probiotics on Caco-2 cell proliferation was identified by MTT colorimetric assay.The 3 probiotics were equally mixed in the same concentration level.The combined toxicity was examined by MIT assay. [Results]The 3 probiotics did not show cytotoxicity at low concentrations（10.2,10.3,10.4 CFU /ml）,but showed significant cytotoxicity at high concentrations（10.5,10.6,10.7,10.8,10.9 CFU/ ml）.The cytotoxicity was enhanced with the increasing of the concentrations.The IC50 of probiotics was 1.90×108 of the Bulgarian bacillus delbrueckii,1.86×109 of Streptococcus thermophilus,1.81×109 of Lactobacillus acidophilus,respectively.The combined toxicity of 3 probiotics on Caco-2 cells showed no significant difference to single cytotoxicity（P0.05）.The effect was due to single probiotic by calculation. [Conclusion]The probiotics of Bulgaria,Streptococcus thermophilus and Lactobacillus acidophilus show cytotoxicity on Caco-2 cell in a dose-dependent manner,but it is not combined toxicity.
出处
《职业与健康》
CAS
2011年第11期1216-1219,共4页
Occupation and Health
基金
吉林省科技发展计划项目(项目编号:20090164)
