摘要
目的该研究应用自行设计合成的纳米金探针通过比色法定量检测CA125,并初步探讨其临床应用的可行性。方法将待检测的CA125捕获抗体连接到纳米金粒子表面制备纳米金探针,经过抗原抗体免疫反应形成纳米金聚集体,通过比色法定量检测CA125。结果成功制备纳米金探针,可快速、简便检测患者血清中CA125的浓度,结果与病理确诊相一致,最低检测限为0.5U/mL,远低于传统的酶联免疫吸附试验。结论该纳米金探针制备简单、快速、容易操作、成本低廉,在临床尤其是基层医院早期检测卵巢癌标志物CA125具有重要的应用价值。
Objective To quantitatively detect CA125 with self-designed synthetic gold nanoparticle probes by the colorimetric immunoassay and to initially investigate the feasibility of clinical application .Methods The capture antibody of detective CA125 was conjugated to the gold nanoparticle surface for preparing the gold nanoparticle probes .The gold nanoparticle aggregation was formed by the antigen-antibody immunoreaction .CA125 was quantitatively detected by the colorimetric immunoassay .Results The gold nanoparticle probes was successfully prepared ,which could detect the serum CA125 content rapidly and conveniently ,the de-tection results were coincident with the pathological diagnosis .The lowest limit of quantitation was 0 .5 U/mL ,which was far lower than that in ELISA .Conclusion This gold nanoparticle probes are simple to be prepared ,rapid and easy to operate with low cost , and have the important application value in clinical early detection of ovarian cancer marker CA 125 ,especially in the primary hospi-tals .
出处
《国际检验医学杂志》
CAS
2014年第15期1976-1978,共3页
International Journal of Laboratory Medicine
基金
广州医科大学青年基金项目(2011A14)
关键词
纳米金探针
卵巢肿瘤
肿瘤标记
生物学
gold nanoparticle probes
ovarian neoplasms
tumor markers,biological
