摘要
表面等离子体激元共振(surface plasmon resonance,SPR)是一种基于芯片表面折射率变化的光学检测技术,具有快速、灵敏、免标记等优点,可实时在线地跟踪固液界面反应,在蛋白及核酸等生物分子检测方面具有广阔的应用前景。但是,传统的SPR检测方法难以检测低分子量、超低浓度的生物分子。纳米金具有密度大、介电常数大、良好的生物相容性等特点,因此,基于纳米金放大的SPR技术既可以保持生物分子的生物活性,又可以有效突破传统SPR中检测限的限制。游离前列腺特异性抗原(free prostate-specific antigenf,-PSA)是一种常用于前列腺癌早期诊断的标志物,但由于其在血清中的浓度非常低,给前列腺癌的早期诊断造成困难。本论文利用基于纳米金放大的SPR技术,设计竞争型免疫模式实现了f-PSA的超灵敏检测。
Surface plasmon resonance（SPR） is an optical analytical technique based on the ability to detect dielectric constant changes induced by molecular adsorption on a noble metal film.SPR is attractive owing to several inherent advantages,such as label-free analysis,rapid and high sensitivity and monitor interfacial reactions at solid-liquid interface.Unfortunately,the inability of conventional SPR to measure extremely small changes in refractive index hinders its application in ultra sensitive detection.Gold nanoparticles（AuNPs） have been used to strengthen the SPR signal due to the ability of their high mass,large dielectric constant,and good biocompatibility.Free prostate-specific antigen（f-PSA） is an effective serum marker currently available for the detection of prostate cancer.Detection of low concentration f-PSA is important to improve the accuracy of the early diagnosis of prostate cancer.In this paper,competitive immunoassay was designed for ultra sensitive determination of f-PSA based on the technology of AuNPs amplified the SPR response.
出处
《中州大学学报》
2011年第5期115-118,共4页
Journal of Zhongzhou University
基金
国家自然科学基金(20773165)
教育部新世纪优秀人才基金(NCET-07-0865)
湖南省杰出青年基金(09JJ1002)
