摘要
设计并制作了微柱型聚二甲基硅氧烷(PDMS)微流控芯片,并在通道表面组装纳米金,固定上皮细胞粘附因子(EpCAM)抗体后用于循环肿瘤细胞(Circulating Tumor Cell,CTC)检测。结果表明,纳米金成功组装在γ-氨丙基三乙氧基硅烷硅烷化的PDMS微柱表面,EpCAM抗体功能化的微流控芯片能有效捕获全血中的模型CTCs,当流速小于40μL/min时,捕获效率大于90%。建立一种简单廉价、捕获效率高的CTCs检测新方法,有望为全血中CTCs的分离提供新的策略。
The PDMS microfluidic chips with micro pillars were designed and fabricated,and then gold nanoparticles were assembled on the surface of PDMS micropillars.After EpCAM antibody was immobilized,the microfluidic chips were used for the detection of model CTCs（circulating tumor cells）in the whole blood.The experimental results showed that gold nanoparticles were successfully assembled on the surface of PDMS miropillars after the microchannels were silanized byγ-aminopropyl triethoxysilane.EpCAM antibody functionalized microfluidic chip can effectively simulated CTCs,and capture efficiency is greater than 90% when the flow velocity is less than 40μL/min.A simple and cheap new method with high capture efficiency of cancer cells was established,which is likely to provide a new strategy for isolation of CTCs from the whole blood.
出处
《武汉理工大学学报》
CAS
CSCD
北大核心
2014年第7期45-49,共5页
Journal of Wuhan University of Technology
基金
中央高校基本科研业务费专项资金资助项目(2013QC018)
国家大学生创新创业计划(201310504037)
关键词
微流控芯片
PDMS微柱
纳米金
循环肿瘤细胞
microfluidic chips
PDMS micropillars
gold nanoparticles
circulating tumor cells
