摘要
目的观察扶正消瘤颗粒对人表皮生长因子受体2(HER-2)阳性乳腺癌细胞SKBR-3增殖和凋亡的影响。方法 SKBR-3细胞以2×106/2 000μL的密度接种到6孔培养板中,共5组。24 h后分别加入1倍、2倍、3倍剂量的扶正消瘤颗粒含药血清,以赫赛汀为阳性对照,空白血清为空白对照。加药后72 h,分别用四甲基偶氮唑蓝显色(MTT)法及流式细胞仪检测SKBR-3细胞的增殖和凋亡。结果 2倍剂量的扶正消瘤颗粒和赫赛汀均可抑制SKBR-3细胞增殖;3种剂量的扶正消瘤颗粒均可促进SKBR-3细胞早期凋亡,2倍剂量组早期凋亡率及总凋亡率最高。结论扶正消瘤颗粒可明显促进乳腺癌SKBR-3细胞凋亡,2倍剂量的扶正消瘤颗粒能抑制乳腺癌SKBR-3细胞增殖。
Objective To observe the effects of Fuzheng Xiaoliu Keli on the proliferation and apoptosis of HER-2-positive breast cancer SKBR-3 cells. Methods SKBR-3 cells were plated into five 6-well-plates at the density of 2×106/ 2 000 μL,also regarded as five groups. After 24 hours,single-dosage,double-dosage,triple-dosage of Fuzheng Xiaoliu Keli contained serum were given to 3 groups of all respectively. The group intervened with Herceptin and blank serum group were used as positive control group and blank control group. After 72 hours of dosing,MTT method and flow cytometry were used to detect the proliferation and apoptosis of KBR-3 cells. Results Double dosage of Fuzheng Xiaoliu Keli and herceptin inhibited the proliferation of SKBR-3 cells. Whatever the dosage,Fuzheng Xiaoliu Keli promoted the apoptosis of SKBR-3 cells. The double-dosage group had the highest early apoptosis rate as well as the total apoptosis rate. Conclusion Fuzheng Xiaoliu Keli can promote the apoptosis of breast cancer SKBR-3 cells significantly. Double-dosage of Fuzheng Xiaoliu Keli can inhibit the proliferation of breast cancer SKBR-3 cells.
出处
《甘肃中医学院学报》
2016年第3期11-15,共5页
Journal of Gansu College of Traditional Chinese Medicine
基金
深圳市科技创新委员会2014年科技研发资金资助(JCYJ20140414170821153)
