摘要
目的探讨康复新体外诱导胃癌BGC-823细胞凋亡及其机制.方法采用四甲基偶氮唑盐(MTT)法测定不同浓度和时间康复新对BGC-823细胞的生长抑制作用;应用流式细胞术进行DNA倍体分析和TUNEL分析,检测康复新对BGC-823细胞增殖和细胞周期的影响以及早期凋亡的情况.结果不同浓度康复新对胃癌BGC-823细胞分别作用24、48、72 h其IC50分别为(17.85±1.06)、(13.76±0.57)和(11.32±0.14)mg/mL(P<0.01),对胃癌BGC-823细胞的抑制作用呈时间和浓度依赖关系;流式细胞术显示胃癌BGC-823细胞出现明显的凋亡峰,随着作用时间的延长,其凋亡率逐渐升高,细胞周期阻滞在G2/M期,S期细胞数大量减少;流式细胞术TUNEL检测结果显示,其作用细胞凋亡和坏死同时存在.结论康复新具有诱导胃癌BGC-823细胞凋亡的作用.
Objective Investigate the apoptosis - inducing activity of Chinese medicine Kangfuxin （KFX） on human peptic carcinoma cell line BGC -823 and its related mechanism. Methods Cell proliferation in different concentration and time was determined by MTT （ Methy thiazolyl tetrazolium） assay. The fluorescence flow cytometry （FCM） DNA assay and TUNEL （Terminal deoxynuxleotidel transferase mediated uridine nucleotide end labeling） were applied to detecting the changes of apoptotic rate at the early and the late apoptosis process, cell proliferation and alteration of cell cycle phase. Results After incubation of BGC - 823 cells with different concentration of Kangfuxin for 24, 48 and 72 hours, the IC50 were （ 17.85 ± 1.06 ）, （ 13.76 ± 0. 57 ） and （ 11.32 ± 0. 14） mg/mL （ P 〈 0. 01 ） . Kangfuxin significantly inhibited the proliferation of BGC - 823 cell and the inhibited effect was dose - and - time - dependent. FCM assay indicated that most of the cells were arrested in G2 - M and the apoptotic peak appeared. During the prolonged incubation time, the apoptosis rate was increased. At the same time, the number of S phase cells decreased. The result of TUNEL indicates that there are apoptosis and necrosis. Conclusion Kangfuxin can induce BGC -823 apoptosis in vitro.
出处
《昆明医学院学报》
2006年第2期5-9,共5页
Journal of Kunming Medical College
基金
国家自然科学基金项目(30560181)
昆明医学院三项基金资助项目(kylc200529)
