摘要
为探讨胰岛素样生长因子 Ⅰ受体 (IGF ⅠR)两亚基 (α,β)和磷酸化酪氨酸蛋白 (P Tyr)在胎儿和成人皮肤中的表达特征 ,用免疫组化方法确定这 3种蛋白在 12例不同胎龄的胎儿和 8例成人皮肤中的定位和表达量的变化规律。结果显示 ,在早期妊娠胎儿的皮肤组织中 ,这 3种蛋白的阳性细胞率很低 ,随着胎儿生长发育 ,IGF ⅠRα、IGF ⅠRβ和P Tyr蛋白表达逐渐增强。在成人皮肤中 ,IGF ⅠRα、β主要分布于表皮细胞的细胞膜上 ,而P Tyr主要定位于表皮细胞和部分成纤维细胞中。这 3种蛋白在不同发育阶段人皮肤组织内都有表达 ,显示它们可能对皮肤的发生。
Localization and expression of two subunits (IGF ⅠRα and IGF ⅠRβ) of insulin like growth factor Ⅰ receptor and phosphorylated tyrosine proteins (P Tyr) in skins at different developmental stages were studied in order to explore their potential biological significance. Immunohistochemistry and pathological methods were used to detect the expression intensity and distribution of IGF ⅠRα, IGF ⅠRβ and P Tyr in skins of 12 fetuses with different gestational ages and 8 adults. The results showed that positive immunohistochemical signals of IGF ⅠRα, IGF ⅠRβ and P Tyr could be found in fetal and adult skins. Along with growth and development of the fetus, the positive cell rates of IGF ⅠRα, IGF ⅠRβ and P Tyr in skins elevated progressively. In adult skins, IGF ⅠR was mainly located in the cell membrane of epidermal cells, while P Tyr was chiefly distributed in epidermal cells and some fibroblasts. These results suggested that IGF ⅠR and its mediating signaling pathway might be involved in cutaneous development at embryonic stage, in cutaneous structure and function maintenance, and in wound healing at postnatal stage.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第11期976-978,W005,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家重大基础研究规划资助项目 (编号G1 9990 542 0 4 )
国家杰出青年科学基金 (编号 3952 50 2 4 )
国家自然科学基金 (编号 30 1 70 996)资助课题
