摘要
Chronic bioenergetic imbalances and inflammation caused by hyperglycemia are obstacles that delay diabetic wound healing.However,it is difficult to directly deliver energy and metabolites to regulate intracellular energy metabolism using biomaterials.Herein,we propose a light-driven bioenergetic and oxygen-releasing hydrogel(PTKM@HG)that integrates the thylakoid membrane-encapsulated polyphenol nanoparticles(PTKM NPs)to regulate the energy metabolism and inflammatory response in diabetic wounds.Upon red light irradiation,the PTKM NPs exhibited oxygen generation and H2O2 deletion capacity through a photosynthetic effect to restore hypoxia-induced mitochondrial dysfunction.Meanwhile,the PTKM NPs could produce exogenous ATP and NADPH to enhance mitochondrial function and facilitate cellular anabolism by regulating the leucine-activated mTOR signaling pathway.Furthermore,the PTKM NPs inherited antioxidative and anti-inflammatory ability from polyphenol.Finally,the red light irradiated PTKM@HG hydrogel augmented the survival and migration of cells keratinocytes,and then accelerated angiogenesis and re-epithelialization of diabetic wounds.In short,this study provides possibilities for effectively treating diseases by delivering key metabolites and energy based on such a light-driven bioenergetic hydrogel.
基金
Fundamental Research Funds for Central Universities(202241010)
Natural Science Fund for Excellent Young Scholars of Shandong Province(ZR202110120029)
Major science and technology projects(110202201020(LS-04))
Open Project of the Key Laboratory for Tobacco Disease and Pest Monitoring and Comprehensive Management in the Tobacco Industry(KLTPMIMT2022-11).
