摘要
为了开发一种针对细菌感染的光热联合化学治疗的生物降解性纳米系统,本研究通过马尔文粒度仪、透射电子显微镜、紫外可见吸收光谱和傅里叶变换红外光谱对纳米抗菌系统进行表征。文章研究了在近红外光照射下不同浓度纳米抗菌系统的光热性能和在不同功率照射下纳米抗菌系统的光热性能以及光热稳定性。通过药物的标准曲线,测定药物的负载率;利用细菌生长曲线法,评估了抗菌剂的抗菌活性;通过透射电子显微镜,检测了纳米抗菌系统的生物降解性。合成的纳米抗菌系统粒径均匀,具有良好的水分散性。在近红外光照射下可重复不衰减升温,在200μg/mL的质量浓度下即可使温度升高22.1℃。与聚多巴胺和硫酸庆大霉素相比,所合成的纳米抗菌系统联合近红外光展现了更为优异的抗菌能力,8μg/mL的PDA-GS对细菌的杀伤率可达到90.76%。合成的纳米抗菌系统展示出了优异的抗菌效果,证实了将光热效应与药物治疗结合的策略在抗菌领域的潜力,为解决细菌感染提供了一种有效途径。
The primary aim of this research is to develop a novel biodegradable nanosystem that leverages both photothermal therapy and chemotherapy for combating bacterial infections.This system seeks to provide a synergistic approach to enhance antibacterial efficacy while ensuring biocompatibility and safety.The study involved a comprehensive characterization of the antibacterial nanosystem using techniques such as malvern particle size analysis,transmission electron microscopy(TEM),UV-visible absorption spectroscopy,and fourier-transform infrared spectroscopy(FTIR).This allowed for an evaluation of the system’s size,morphology,dispersion in aqueous solutions,and functional groups.The photothermal performance of the nanosystem was assessed under near-infrared(NIR)light irradiation at various concentrations and power levels,including an analysis of its photothermal stability.The drug loading efficiency was determined using the drug’s standard curve.Antibacterial efficacy was evaluated through bacterial growth curve assays,providing insights into the antimicrobial activity of the system.Additionally,the biodegradability of the nanosystem was investigated using TEM to observe structural changes over time.The synthesized nanosystem demonstrated uniform particle size distribution and excellent water dispersibility.Under NIR irradiation,the system exhibited consistent and significant temperature increases,with a noticeable rise of 22.1℃ at a concentration of 200μg/mL.Remarkably,when compared to controls such as polydopamine and gentamicin sulfate,the developed nanosystem,under NIR illumination,showed enhanced antibacterial performance,achieving a bacterial kill rate of up to 90.76%at a lower concentration of 8μg/mL PDA-GS.This indicates a superior antibacterial capability facilitated by the combined effects of photothermal therapy and chemotherapy.The research successfully demonstrates the development of a biodegradable nanosystem that combines photothermal and chemotherapeutic strategies for the effective treatment of
作者
王颖
WANG Ying(Henan Technician College of Medical and Health,Kaifeng 475000,China)
出处
《药物生物技术》
CAS
2024年第4期361-365,共5页
Pharmaceutical Biotechnology
基金
《诊断试剂生产工》国家职业技能标准制定(No.SYHZW201814)。
关键词
聚多巴胺
硫酸庆大霉素
抗菌
光热治疗
生物可降解
联合治疗
Polydopamine
Gentamicin sulfate
Antibacterial
Photothermal therapy
Biodegradable
Combination therapy
