摘要
实体瘤中普遍存在乏氧现象,是导致肿瘤对非手术治疗手段抗拒性增加,降低药物疗效的重要因素。针对这一问题,本研究采用简单的两相界面法制备了一种小尺寸(65nm)、单分散、生物稳定性良好的可共载抗癌药物盐酸阿霉素(DOX)和载氧蛋白血红蛋白(Hb)的树枝状介孔硅纳米颗粒(DMSNs)。利用扫描电子显微镜(SEM)、透射电子显微镜(TEM)、动态光散射仪(DLS)和氮气吸附–脱附仪等对材料进行表征。结果表明,合成的DMSNs纳米颗粒粒径均一、分散性良好,具有较大的比表面积(654.52 m^2/g)和孔容(1.26cm^3/g)以及两套孔道结构(直径2.7nm和5.4～6.8 nm)。更重要的是,树枝状介孔层的孔径仅需改变三乙醇胺(TEA)的用量即可调节。药物释放、流式细胞术、激光共聚焦以及细胞毒性等相关实验结果表明,DMSNs可同时装载DOX与Hb,且具有较高的药物释放能力(75.6%)和持久的释放性能(48h)。载入血红蛋白后,其IC_(50)为20.6μg/mL,能够有效提高抗癌药物DOX的细胞致死率。因此,这种小尺寸的树枝状介孔硅球在药物传输和肿瘤治疗方面具有潜在的应用价值。
Tumor hypoxia is a ubiquitous factor to cause the therapeutic resistance of drug or other treatments. To solve this problem, a facile biophase stratification approach was developed to fabricate dendritic mesoporous silica nanoparticles （DMSNs） with small particle size of 65 nm, high dispersity and excellent biostability through simply controlling the oil-water interracial reaction. The resultant DMSNs were characterized by various techniques such as scanning electron microscope （SEM）, transmission electron microscope （TEM）, dynamic light scattering （DLS）, and N2 adsorption-desorption analysis, etc. It is verified that DMSNs possess two types of pore size （2.7 nm and 5.4-6.8 nm）, high specific surface （654.52 m^2/g） and pore volume （1.26 cm^3/g）, which are capable to co-load DOX and Hb, simul- taneously. Moreover, the mesopore size in the dendritic mesoporous layer could be tuned by changing the amounts of triethanolamine （TEA）. The testing results from drug release, flow cytometry histograms, confocal laser scanning mi-croscopy, and cell cytotoxicity demonstrate that DMSNs possess high efficiency of drug release （75.6%）, durable releasing period （48 h） and significantly enhanced cell lethality （IC50= 20.6 μg/mL）. These data demonstrate that such kind of dentritic mesoporous silica naoparticles has great potentials in drug delivery and tumor chemotherapy.
作者
潘珊
李永生
施剑林
PAN Shan;LI Yong-Sheng;SHI Jian-Lin(School of Material Science and Engineering,East China University of Science and Technology,Shanghai 200237,China;Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai 200050,China)
出处
《无机材料学报》
SCIE
EI
CAS
CSCD
北大核心
2018年第10期1097-1102,共6页
Journal of Inorganic Materials
基金
国家自然科学基金(51461165202
51472085)~~
关键词
乏氧
小尺寸
树枝状介孔硅纳米颗粒
药物传输
rumor hypoxia
small size
dendritic mesoporous silica nanoparticles
drug delivery
