摘要
藏羊产于青藏高原及其毗邻地区,外部特征非常突出,杂色藏羊约占总群体的90%,被毛纯白和纯黑个体很少。为研究ASIP基因的SNPs多态性与藏羊毛色遗传之间的关系,本研究采用混池测序及PCR-SSCP法对ASIP基因7个外显子在高原型、欧拉型、扎什加型和黑藏羊4种类型藏羊群体中的突变位点进行了检测。结果共发现5个位点存在突变,分别为外显子3 g.49396C>G和外显子7的g.76594G>C、g.76715T>A、g.76732C>G和g.76811C>A。其中突变位点g.76715T>A碱基颠换引起第126位的半胱氨酸转变为丝氨酸(C126S)。而突变位点g.49396C>G在4个藏羊类型中都存在CC、CG、GG 3种基因型,该突变位点在高原型、欧拉型、扎什加型和黑藏羊群体中的最小突变概率分别为0.416、0.234、0.261、0.307,多态信息含量分别为0.485、0.358、0.386、0.424,都为中度多态。除欧拉型藏羊外,高原型、黑藏羊、扎什加型3个群体处于Hardy-Weinberg平衡状态。研究结果显示,ASIP基因g.49396C>G和g.76715T>A错义突变应是调控藏羊毛色遗传的重要位点,可为藏羊毛色的选育提供潜在的遗传标记。
Tibetan sheep are mainly distributed in the Qinghai-Tibet Plateau and its adjacent areas,with a distinctive external appearance.About 90%of the total population are mixed-colored,while pure white and pure black individuals are rare.In order to study the relationship between SNPs of ASIP gene and the heredity of Tibetan wool color,this study used pooled sequencing and PCR-SSCP methods to detect the polymorphisms of seven exons of the ASIP gene in four ecological types of Tibetan sheep populations,including the plateau type,the Oula type,the Zhashijia type,and the black Tibetan sheep.Results showed that five mutation sites were found,including g.49396C>G in exon 3 and g.76594G>C,g.76715T>A,g.76732C>G,and g.76811C>A in exon 7.The mutation site g.76715T>A caused the conversion of cysteine to serine at position 126(C126S).The mutation site g.49396C>G had three genotypes(CC,CG,and GG)in all four ecological types of Tibetan sheep,with the minimum mutation probabilities of 0.416,0.234,0.261,and 0.307,and the polymorphic information contents of 0.485,0.358,0.386,and 0.424,respectively,indicating moderate polymorphism.Except for the Oula type,the plateau type,black Tibetan sheep,and Zhashijia type were all in Hardy-Weinberg equilibrium.The results showed that the g.49396C>G and g.76715T>A missense mutations in ASIP gene are important sites regulating the inheritance of Tibetan wool color,which can serve as potential genetic markers for the breeding of Tibetan wool color.
作者
张耀
王啟婷
张存芳
多杰才旦
蔡伟杰
郝佳慧
覃敏欣
ZHANG Yao;WANG Qiting;ZHANG Cunfang;Duojiecaidan;CAI Weijie;HAO Jiahui;QINMinxin(State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining,Qinghai 810016,China;College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai 810016,China;Huangyuan County Animal Husbandry and Veterinary Station,Huangyuan,Qinghai 812199,China;Animal Disease Prevention and Control Center of Hainan Tibetan Autonomous Prefecture,Gonghe,Qinghai 813099,China)
出处
《家畜生态学报》
北大核心
2024年第10期15-21,共7页
Journal of Domestic Animal Ecology
基金
青海省应用基础研究项目(2021-ZJ-728)。
