摘要
红榄李(Lumnitzera littorea)自然繁殖困难,野外种群濒临灭绝。为保护红榄李种质资源,本研究以其幼苗叶片为外植体,基于MS培养基探究了不同次氯酸钠溶液(NaClO)浓度和消毒时间、不同光照强度和植物生长调节剂浓度对其消毒效果和愈伤组织诱导和增殖的影响。结果表明:50 g·L^(-1)NaClO消毒10min效果最好,外植体污染率和重度褐化率降至15.5%和35.5%。暗处理下外植体愈伤诱导效果最好,愈伤诱导率达19.4%。接种前对叶片外植体中部进行切割并在0.1 g·L^(-1)NAA+0.05 g·L^(-1)6-BA高浓度溶液中浸泡30~40 min,然后接种于1.2 mg·L^(-1)NAA+0.1 mg·L^(-1)6-BA培养基中黑暗培养30 d,愈伤诱导率可达50.0%。1.2 mg·L^(-1)IAA+0.1 mg·L^(-1)6-BA和1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA最利于愈伤组织增殖,外植体干鲜重达最大值。
Lumnitzera littorea is difficult to reproduce naturally,and the wild population is on the verge of extinction.In order to protect the germplasm resources,this study investigated the sterilization effects of different NaClO solution concentrations and sterilization time on seedlings leaves,light intensities and plant growth regulator concentrations on callus induction and proliferation of L.littorea based on MS medium.The results showed that:50 g·L^(-1) NaClO sterilized for 10 min had the best effect,which decreased contamination rate and severe browning rate of explants to 15.5%and 35.5%,respectively.The callus induction effect of the explants in the dark was the best,and the callus induction rate was 19.4%.Before inoculation,the explants of the middle part leaf were hurt and immersed in 0.1 g·L^(-1) NAA+0.05 g·L^(-1)6-BA solution for 30-40 min,and then inoculated in 1.2 mg·L^(-1) NAA+0.1 mg·L^(-1)6-BA medium cultured in the dark for 30 days which caused rate of the callus induction up to 50.0%. 1.2 mg·L^(-1) IAA+0.1 mg·L^(-1) 6-BAand 1.0 mg·L^(-1) 6-BA+0.1 mg·L^(-1) NAA were the best medium for callu proliferation, and the dry and freshweight of explants reached the maximum.
作者
李小朋
王佳
赵秀芳
佘忠明
刘厚诚
LI Xiaopeng;WANG Jia;ZHAO Xiufang;SHE Zhongming;LIU Houcheng(College of Horticulture,South China Agricultural University,Guangzhou 510642,China;Lingnan Ecological Culture Travel Co.Ltd.,Dongguan,Guangdong 523125,China;Huashengfeng Ecology(Shenzhen)Co.,Ltd.,Shenzhen,Guangdong 518101,China;Peking University Shenzhen Graduate School,Shenzhen,Guangdong 518057,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2024年第9期1453-1460,共8页
Plant Physiology Journal
基金
广东省重点领域研发计划(2019B020214005)
企业自主研发项目(KY0093)
深圳市科技计划(KCXST20221021111416039)。
关键词
红榄李
幼苗叶片
暗处理
愈伤组织
Lumnitzera littorea
seedling leaf
dark treatment
callus
