摘要
以伊藤杂种‘和谐’(Itoh hybrids ‘He Xie’)的鳞芽为材料建立离体快繁技术体系,可克服传统方法繁育较慢的缺点,加速伊藤杂种优良品种的繁育推广。采用单因子试验设计,分别探究不同灭菌时间、不同植物生长调节剂浓度、不同根诱导时间以及不同生根苗等级对‘和谐’组培苗启动、增殖、生根和驯化效果的影响。结果表明,用2%次氯酸钠溶液的最佳灭菌时间为12分钟,鳞芽污染率为9.09%;最佳初始培养基配方为MS+1.5 mg·L^(-1)6-BA+0.2 mg·L^(-1)GA_(3)+0.5 mg·L^(-1)AgNO_(3);最佳增殖培养基配方为MS+450 mg·L^(-1)CaCl_(2)+0.5 mg·L^(-1)6-BA+0.2 mg·L^(-1)IBA+0.2 mg·L^(-1)GA_(3)+0.5 mg·L^(-1)AgNO_(3),增殖系数为3.3;无根苗在根诱导培养基1/2MS+1.0 mg·L^(-1)腐胺+2.0 mg·L^(-1)IBA上,经4℃低温暗培养8天后常温光照培养30天,再转入根形成培养基1/2MS+1.0 g·L^(-1)AC,培养20天生根率达66.7%;苗移栽的基质为珍珠岩:蛭石:草炭土=1:1:1 (v/v/v),移栽60天后,一级苗成活率最高为52.0%,二级苗和三级苗则大部分死亡,表明生根质量对移栽成活至关重要。
An in vitro rapid propagation system using scale buds of Itoh hybrids‘He Xie’can overcome the shortcomings of the slow traditional breeding methods and promote the adoption of the excellent Itoh hybrid varieties.In this study,we used the buds of‘He Xie’as explants to investigate the effects of various factors including disinfection time,plant growth regulators(PGRs)concentration and root induction time,and different rooted seedling grades on the initiation,proliferation,rooting and domestication of‘He Xie’with one-way experimental design.Our results showed:the optimal disinfection time of buds by 2%sodium hypochlorite solution was 12 min,with a contamination rate of 9.09%;the optimal initial culture medium was MS+1.5 mg·L^(-1)6-BA+0.2 mg·L^(-1) GA_(3)+0.5 mg·L^(-1) AgNO_(3);the optimal proliferation culture medium was MS+450 mg·L^(-1) CaCl_(2)+0.5 mg·L^(-1)6-BA+0.2 mg·L^(-1) IBA+0.2 mg·L^(-1) GA_(3)+0.5 mg·L^(-1) AgNO_(3) with a proliferation rate of 3.3.Rootless seedlings were cultured on root induction medium 1/2MS+1.0 mg·L^(-1) putrescine+2.0 mg·L^(-1) IBA for 8 d at 4℃ in dark and then 30 d at room temperature under light,and finally transferred to root formation medium 1/2MS+1.0 g·L^(-1) AC for 20 d,a rooting rate of 66.7%was observed.The rooted seedlings were transplanted on a growing matrix of perlite:vermiculite:charcoal soil=1:1:1(v/v/v)for 60 d,with the highest transplant survival rate of 52.0%being observed for first-grade rooted seedlings,but most of the second-and third-grade seedlings being dead,suggesting that the quality of rooting is critical for transplant survival.
作者
康敏
张美莹
齐秀双
佟宁宁
李旸
舒庆艳
刘政安
吕长平
彭丽平
Min Kang;Meiying Zhang;Xiushuang Qi;Ningning Tong;Yang Li;Qingyan Shu;Zheng’an Liu;Changping Lü;Liping Peng(College of Horticulture,Hunan Agricultural University,Changsha 410128,China;College of Landscape Architecture and Art Design,Hunan Agricultural University,Changsha 410128,China;Hunan Provincial Engineering and Technology Research Center for Breeding and Utilization of Central-subtropical High-quality Flowering Trees,Hunan Agricultural University,Changsha 410128,China;National Key Laboratory of Plant Diversity and Special Economic Crops,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China;National Botanical Garden,Beijing 100093,China;Tian Xiang Yuan(Liaoning)Biotechnology Co.Ltd.,Huludao 125300,China)
出处
《植物学报》
CAS
CSCD
北大核心
2024年第3期441-451,共11页
Chinese Bulletin of Botany
基金
国家自然科学基金青年科学基金(No.32201609)
中国科学院植物研究所企业横向项目(No.E0119C6001)。
关键词
伊藤‘和谐’
启动与增殖
生根
鳞芽
移栽驯化
Itoh‘He Xie’
initiation and proliferation
rooting
scale buds
transplant domestication
