摘要
目的探讨铜死亡相关基因铁氧还原蛋白1(FDX1)和二氢硫辛酰胺转乙酰基酶(DLAT)在子宫内膜癌中的表达及临床意义。方法选取2020年9月至2023年8月徐州市中心医院收治的110例子宫内膜癌患者作为研究对象,术中取子宫内膜癌组织和癌旁组织。采用实时荧光定量聚合酶链反应法检测癌组织及癌旁组织中FDX1和DLAT表达水平,分析子宫内膜癌组织中FDX1和DLAT表达水平与临床病理特征的关系,采用Kaplan-Meier生存曲线分析FDX1和DLAT表达与患者3年预后的关系,采用Cox回归分析子宫内膜癌患者预后的影响因素。结果子宫内膜癌组织FDX1表达水平低于癌旁组织,DLAT表达水平高于癌旁组织(P<0.05)。国际妇产科联盟(FIGO)分期Ⅲ~Ⅳ期、有淋巴结转移、肌层浸润≥1/2、有脉管浸润的FDX1低表达和DLAT高表达患者比例高于FIGO分期Ⅰ~Ⅱ期、无淋巴结转移、肌层浸润<1/2、无脉管浸润患者(P<0.05)。FDX1低表达患者3年生存率低于FDX1高表达患者(P<0.05);DLAT高表达患者3年生存率低于DLAT低表达患者(P<0.05)。FIGO分期、DLAT是子宫内膜癌患者3年预后死亡的独立危险因素,FDX1是3年死亡的保护因素(P<0.05)。结论子宫内膜癌组织中FDX1低表达,DLAT高表达,两者与子宫内膜癌患者临床病理特征及预后相关,可能成为子宫内膜癌诊断、治疗和预后改善的新的作用靶点。
Objective To investigate the expression and clinical significance of cuproptosis related genes such as ferredoxin 1(FDX1)and dihydrolipoamide transacetylase(DLAT)in endometrial cancer.Methods A total of 110 patients with endometrial cancer admitted to Xuzhou Central Hospital from September 2020 to August 2023 were collected as research objects,and the endometrial cancer tissue and adjacent tissues were taken during surgery.Quantitative real-time polymerase chain reaction method was applied to detect the expression levels of FDX1 and DLAT in endometrial cancer tissue and adjacent tissues,and the relationship between the expression levels of FDX1 and DLAT in the endometrial cancer tissues and the clinicopathological characteristics was analyzed.Kaplan-Meier survival curve was applied to analyze the relationship between the expression levels of FDX1 and DLAT and the 3-year prognosis of patients.Cox regression was applied to analyze the prognostic factors of endometrial cancer patients.Results The expression level of FDX1 in the endometrial cancer tissue was lower than that in the adjacent tissue,while the expression level of DLAT was higher than that in the adjacent tissue(P<0.05).The proportions of low FDX1 expression and high DLAT expression in patients with international federation of gynecology and obstetrics(FIGO)stagesⅢ-Ⅳ,lymph node metastasis,myometrial infiltration≥1/2,and vascular infiltration were higher than those in patients with FIGO stagesⅠ-Ⅱ,no lymph node metastasis,myometrial infiltration<1/2,and no vascular infiltration(P<0.05).The 3-year survival rate of patients with low FDX1 expression was lower than that of patients with high FDX1 expression(P<0.05).The 3-year survival rate of patients with high DLAT expression in endometrial cancer tissue was lower than that of patients with low DLAT expression(P<0.05).FIGO staging and DLAT were the independent risk factors for 3-year mortality in endometrial cancer patients,while FDX1 was a protective factor for 3-year mortality(P<0.05).Conclusions F
作者
王维奇
陶巍
张晶波
贺磊磊
郭琳琳
张蓓
WANG Weiqi;TAO Wei;ZHANG Jingbo;HE Leilei;GUO Linlin;ZHANG Bei(Master Degree Candidate of Obstetrics and Gynecology Enrolled in 2007,Graduate School,Xuzhou Medical University,Xuzhou 221004,Jiangsu,China;The Affiliated Xuzhou Clinical College of Xuzhou Medical University,Department of Obstetricsand Gynecology,Xuzhou Central Hospital,Xuzhou 221009,Jiangsu,China)
出处
《中国性科学》
2024年第10期91-95,共5页
Chinese Journal of Human Sexuality
基金
江苏省社会发展面上项目(BE2019636)
江苏省中医药科技发展计划项目(ZT002114)。
