摘要
目的分析微小核糖核苷酸(miRNA)-125b、miR-29a、miR-425-5p在子宫内膜癌患者血清中相对表达水平变化及临床意义。方法收集2019年5月至2021年3月绵阳市中心医院收治的124例子宫内膜癌患者作为子宫内膜癌组,另选择同期体检的124例健康女性作为健康对照组。应用实时荧光定量PCR检测两组血清miR-125b、miR-29a、miR-425-5p相对表达水平。比较两组血清miR-125b、miR-29a、miR-425-5p相对表达水平,分析其与肿瘤临床分期、淋巴结转移的关系,通过Pearson相关分析miR-125b、miR-29a、miR-425-5p之间的相关性。结果子宫内膜癌组血清miR-425-5p相对表达水平明显高于健康对照组,血清miR-125b、miR-29a相对表达水平明显低于健康对照组(P<0.05);Ⅰ~Ⅱ期子宫内膜癌患者血清miR-125b、miR-29a相对表达水平高于Ⅲ~Ⅳ期患者,血清miR-425-5p相对表达水平低于Ⅲ~Ⅳ期患者(P<0.05);中高分化子宫内膜癌患者血清miR-125b、miR-29a水平高于低分化患者,血清miR-425-5p表达水平低于低分化患者(P<0.05);淋巴结转移的子宫内膜癌患者miR-125b、miR-29a相对表达水平高于无淋巴结转移患者,血清miR-425-5p相对表达水平低于无淋巴结转移患者(P<0.05);浅肌层浸润的子宫内膜癌患者血清miR-125b、miR-29a相对表达水平高于深肌层浸润患者,血清miR-425-5p相对表达水平低于深肌层浸润患者(P<0.05)。Pearson相关分析结果显示,子宫内膜癌患者miR-425-5p与miR-125b、miR-29a均呈负相关(r=-0.419、-0.436,P<0.05)。结论miR-425-5p在子宫内膜癌患者血清中相对表达水平升高,miR-125b、miR-29a在子宫内膜癌患者血清中相对表达水平降低,且其与临床分期、淋巴结转移密切相关,可能作为子宫内膜癌诊断、治疗的新型靶点。
Objective To analyze the changes of relative expression of miR-125b,miR-29a and miR-425-5p in serum of patients with endometrial cancer and their clinical significance.Methods A total of 124 patients with endometrial cancer admitted to Mianyang Central Hospital from May 2019 to March 2021 were selected as endometrial cancer group,and another 124 healthy women who underwent physical examination during the same period were selected as healthy control group.The relative expression levels of miR-125b,miR-29a and miR-425-5p in serum of the two groups were detected by real-time fluorescence quantitative PCR.The relative expression levels of miR-125b,miR-29a and miR-425-5p in serum of the two groups were compared to analyze their relationship with clinical stage of tumor and lymph node metastasis.The correlation between miR-125b,miR-29a and miR-425-5p was studied by Pearson correlation analysis.Results The relative expression level of miR-425-5p in endometrial cancer group was significantly higher than that in healthy control group,and the expression levels of miR-125b and miR-29a in endometrial cancer group were significantly lower than that in healthy control group(P<0.05).The relative expression levels of miR-125b and miR-29a in patients with metastatic endometrial cancer ofⅠ-Ⅱstagge were higher than those in patients with metastatic endometrial cancer ofⅢ-Ⅳstage,and the relative expression levels of miR-425-5p were lower than those in patients with metastatic endometrial cancer ofⅢ-Ⅳstagge(P<0.05).The relative expression levels of serum miR-125b and miR-29a in moderately and highly differentiated endometrial cancer patients were higher than those in poorly differentiated patients,and the relative expression level of serum mir-425-5p was lower than that in poorly differentiated patients.The relative expression levels of miR-125b and miR-29a in endometrial cancer patients with lymph node metastasis were higher than those in patients without lymph node metastasis,and the relative expression levels of miR-42
作者
邱菊
严娅
龚净娴
田春艳
QIU Ju;YAN Ya;GONG Jingxian;TIAN Chunyan(Department of Obstetrics and Gynecology,Mianyang Hospital Affiliated to the School of Medicine of the University of Electronic Science and Technology/Mianyang Central Hospital,Mianyang,Sichuan 621000,China;Department of Gastroenterology,West China Hospital,Sichuan University/West China School of Nursing,Sichuan University,Chengdu,Sichuan 610000,China)
出处
《国际检验医学杂志》
CAS
2022年第9期1120-1123,1128,共5页
International Journal of Laboratory Medicine
