摘要
目的:探讨长链非编码RNA(long non-coding RNA,lncRNA)肌动蛋白纤维相关蛋白-1反义RNA1(actin filamentassociated protein 1-antisense RNA1,AFAP1-AS1 RNA1)在胃癌组织中的表达情况及其临床意义。方法:采用Real-time PCR方法检测2010年1月至2014年12月宜昌市第二人民医院及三峡大学仁和医院手术切除的274例胃癌组织及相应癌旁正常胃组织中AFAP1-AS1的表达,并分析AFAP1-AS1表达量与临床及病理特征的关系。结果:AFAP1-AS1在胃癌组织比癌旁正常组织显著高表达(P<0.01)。AFAP1-AS1在早期胃癌组织中平均表达量明显低于进展期胃癌(P<0.01)。在不同病理类型胃癌中,AFAP1-AS1的表达量没有差异(P>0.05)。AFAP1-AS1表达量和胃癌淋巴侵袭或远处转移密切相关,在伴有淋巴结侵袭或远处转移的胃癌组织中,AFAP1-AS1明显高表达(P<0.01)。结论:AFAP1-AS1可能是胃癌发生发展的一个重要因素;有望成为新的胃癌预后标志物,用于胃癌的临床诊断和预后判断。
Objective：To explore expression of actin filament-associated protein 1-antisense RNA1 （AFAP1-AS1）,long non-coding RNA （lncRNA）,in the gastric carcinoma tissues and its clinical significance.Methods：Using real-time PCR assay,expressions of AFAP1-AS1 in 274 cases of gastric carcinoma tissues and their corresponding adjacent normal gastric tissues which collected from the patients with gastric carcinoma who were hospitalized in the 24 People Hospital of Yichang City and Renhe Hospital of Sanxia University for operative treatment during January 2010 to December 2014 were detected.And relationship between the expression of AFAP1-AS1 and clinicopathological features of the patients with gastric carcinoma was analyzed.Results：Expression of AFAP1-AS1 in the gastric carcinoma tissue was obviously higher than that in the adjaceut normal gastric tissues.Mean expression amount of AFAP1-AS1 in the early gastric carcinoma tissue was significantly lower than that in the advanced gastric carcinoma tissue （P ＜ 0.01）.Among the gastric carcinoma tissues of various pathological types,expression amounts of AFAP1-AS1 were not significantly different （P ＞ 0.05）.Expression amounts of AFAP1-AS1 were closely correlated with lymphatic invasion and distant metastasis of the gastric carcinoma.Expression amounts of AFAP1-AS1 in the gastric carcinoma tissue with local invasion and distant metastasis were obviously higher than those in the gastric carcinoma tissues without local invasion and distant metastasis （P ＜ 0.05）.Conclusion：AFAP1-AS1 could be an important regulatory factor in the gastric carcinogenesis and development.It could be expected to become a novel marker for judging prognosis of the gastric carcinoma and used for clinical diagnosis and prognosis evaluation of the gastric carcinoma.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2017年第3期290-294,共5页
Chinese Journal of Cancer Biotherapy
关键词
长链非编码RNA
肌动蛋白纤维相关蛋白-1反义RNA1
胃癌
long non-coding RNA （lncRNA）
actin filament-associated protein 1-antisense RNA1 （AFAP1-AS1）
gastric cancer
