摘要
以葛仙米多糖提取率为考察指标,在单因素试验的基础上,通过响应面试验对超声波结合酶法提取葛仙米多糖的工艺条件进行优化,并对葛仙米粗多糖进行纯化获得醇溶性葛仙米多糖组分,分别考察纯化后各多糖组分在酒体中的抗疲劳作用;最后,使用高效凝胶渗透色谱仪对得到的抗疲劳活性组分的分子质量范围进行测定。结果表明,葛仙米多糖最佳提取工艺条件为复合酶添加量2.0%、酶解pH4.9、酶解时间2.0 h、酶解温度50℃、超声功率163 W、料液比1:200 g/mL,在此最优条件下,葛仙米多糖提取率为(38.57±0.47)%。使用DEAE Seplife A25葡聚糖凝胶层析柱对葛仙米粗多糖进行分离纯化,分别得到2个多糖组分:GXM-1和GXM-2,且分子质量分布范围分别集中于(7×10^(5)~60×10^(5))u和(9×10^(3)~6×10^(4))u。抗疲劳结果表明:中剂量的GXM-1和GXM-2均具有较强的抗疲劳活性。研究结果可为葛仙米多糖功能性食品的开发和利用提供数据支撑。
The extraction rate of polysaccharides from Nostoc sphaeroides Küte was used as the index,and on the basis of single factor experiments,the process conditions for extracting polysaccharides from Nostoc sphaeroides Küte by ultrasound combined with enzyme method were optimized through response surface methodology.The crude polysaccharides of Nostoc sphaeroides Küte was purified to obtain alcohol soluble components and the anti-fatigue effects of each purified polysaccharide component were investigated in wine body,respectively.Finally,the molecular weight range of the obtained components with anti-fatigue activity was determined by high-performance gel exclusion chromatography.The results showed that the optimal extraction rate of polysaccharides from Nostoc sphaeroides Küte was(38.57±0.47)%when the addition amount of composite enzyme was 2.0%,enzymatic hydrolysis pH was 4.9,enzymatic hydrolysis time was 2.0 h,enzymatic hydrolysis temperature was 50℃,ultrasonic power was 163 W,and solid-liquid ratio was 1:200 g/mL.The crude polysaccharide from Nostoc sphaeroides Küte was separated and purified by DEAE Seplife A25 glucan gel chromatography column,and two polysaccharide components were obtained:GXM-1 and GXM-2,respectively.The molecular weight distribution ranges were concentrated in(7×10^(5)-60×10^(5))u and(9×10^(3)-6×10^(4))u,respectively.The results of the anti-fatigue study showed that both GXM-1 and GXM-2 at a medium dose had a strong anti-fatigue activity.This study can provide data support for the development and utilization of functional foods containing the polysaccharides from Nostoc sphaeroides Küte.
作者
陈阳
潘凯进
石万银
李慧
徐剑
杜培艳
王喆
CHEN Yang;PAN Kaijin;SHI Wanyin;LI Hui;XU Jian;DU Peiyan;WANG Zhe(Hubei Provincial Key Laboratory of Quality and Safety of Traditional Chinese Medicine Health Food,Jing Brand Co.,Ltd.,Daye 435100,China)
出处
《食品科技》
CAS
北大核心
2024年第8期200-209,共10页
Food Science and Technology
基金
湖北省科技厅自然科学基金联合基金项目(2022CFD076)。
关键词
葛仙米
超声波结合酶法
分离纯化
抗疲劳活性
分子质量范围
Nostoc sphaeroides Kute
ultrasound combined with enzyme method
isolation
anti-fatigue activity
molecular weight range
