摘要
以油莎豆粕为原料,采用超声波辅助法提取水溶性多糖,通过单因素试验与正交试验确定最佳提取工艺,并对多糖结构和抗氧化性进行研究。结果表明,油莎豆粕水溶性多糖最佳提取工艺条件为料液比1∶30(g/mL),超声时间6 min,超声功率450 W。在此工艺条件下,油莎豆粕水溶性多糖提取率为30.49%。红外光谱表明,提取的油莎豆粕水溶性多糖呈现出典型的以α-吡喃糖为骨架的多糖特征吸收峰。抗氧化性结果显示,多糖浓度为6 mg/mL时,DPPH自由基和超氧阴离子自由基清除率分别为98.7%、96.8%;多糖浓度为1.5 mg/mL时,羟自由基清除率为98.0%。
The water-soluble polysaccharides were extracted from Cyperus esculentus meals by the ultrasonic-assisted method.The optimal extraction process was determined by the single factor experiment and orthogonal test,and the structure and antioxidant activity of polysaccharides were investigated.The results showed that the optimal extraction process conditions for water-soluble polysaccharides from C.esculentus meals were as follows:solid-liquid ratio of 1∶30(g/mL),ultrasonic time of 6 min,and ultrasonic power of 450 W.Under the above process conditions,the extraction rate of water-soluble polysaccharides from C.esculentus meals was 30.49%.Infrared spectra indicated that the extracted polysaccharides exhibited typical absorption peaks ofα-pyranose as the backbone.The results of the antioxidant experiment showed that when the concentration of polysaccharides was 6 mg/mL,the scavenging rates against DPPH free radicals and superoxide anion radicals were 98.7%and 96.8%,respectively.When the concentration of polysaccharides was 1.5 mg/mL,the scavenging rate against hydroxyl radicals was 98.0%.
作者
吴修利
徐雷
谢英
司美双
WU Xiu-li;XU Lei;XIE Ying;SI Mei-shuang(College of Food Science and Engineering,Changchun University,Changchun 130022,Jilin,China)
出处
《食品研究与开发》
CAS
北大核心
2022年第16期81-87,共7页
Food Research and Development
基金
国家级大学生创新创业训练计划项目(2020GJ035)
吉林省科技发展计划项目(20200502003NC)
长春大学科研培育项目(ZKQ201909)。
关键词
油莎豆粕
水溶性多糖
超声波辅助法
正交试验
抗氧化性
Cyperus esculentus meals
water-soluble polysaccharides
ultrasonic-assisted method
orthogonal test
antioxidation activity
