摘要
为探究滇皂角米多糖碱提工艺,以多糖提取率为指标,通过单因素试验考察液料比、NaOH浓度、提取温度及提取时间对滇皂角米多糖提取率的影响,结合Box-Behnken响应面试验对提取工艺进行优化,并对多糖进行体外抗氧化探究。结果表明,滇皂角米多糖的碱提最佳工艺:液料比220∶1(mL/g)、NaOH溶液浓度0.16 mol/L、提取温度51℃、提取时间90 min,在该条件下滇皂角米多糖的提取率为(73.64±0.56)%。此外,滇皂角米多糖具有一定的体外抗氧化能力,清除DPPH自由基、ABTS^(+)自由基的IC_(50)值分别为10.87、6.67 mg/mL。
To investigate the alkalinity extraction technology of polysaccharides from the seeds of Gleditsia japonica,the extraction yields were used as indicators for the effects of four factors.These included the liquid-solid ratio NaOH solution concentration,extraction temperature,and extraction time on the efficacy of polysaccharides from the seeds of Gleditsia japonica,using single-factor tests.Based on the experimental results,Box-Behnken response surface methods were conducted to optimize the extraction process.In addition,the antioxidant capacities (in vitro) of the extracts were analyzed.The results showed that the optimum extraction conditions included:a liquid-solid ratio of 220∶1 (mL/g),alkali concentration of 0.16 mol/L,extraction temperature of 51 ℃,and extraction time of 90 min.Under these conditions,the extraction yield was (73.64±0.56) %.Moreover,the polysaccharides possessed certain antioxidant capacities in vitro.The IC_ (50) values for scavenging DPPH and ABTS~+free radicals were 10.87 mg/mL and 6.67 mg/mL,respectively.
作者
徐瑾钰
彭小伟
阚欢
赵平
全伟
刘云
XU Jinyu;PENG Xiaowei;KAN Huan;ZHAO Ping;QUAN Wei;LIU Yun(School of Life Sciences,Southwest Forestry University,Kunming 650224,Yunnan,China;School of Materials and Chemical Engineering,Southwest Forestry University,Kunming 650224,Yunnan,China;Yunnan Province Rural Science and Technology Service Center,Kunming 650021,Yunnan,China)
出处
《食品研究与开发》
CAS
北大核心
2023年第14期112-118,共7页
Food Research and Development
基金
大学生创新创业训练计划项目(202010677003)。
关键词
滇皂角米
多糖
碱提法
工艺优化
抗氧化活性
seed of Gleditsia japonica
polysaccharide
alkalinity extraction methods
process optimization
an-tioxidant activity
