摘要
GeBP基因家族编码的转录因子可通过调节表皮毛的生长与分化影响植物的发育,增强植株在不利环境中的存活能力。为探究GeBP基因在蓖麻基因组中的分布与特征基因RcGeBP2的功能,本研究鉴定了蓖麻的全部GeBP基因,采取RT-PCR技术克隆RcGeBP2基因,分析其分子特征包括生物信息学、亚细胞定位和表达模式。试验表明,蓖麻的基因组中共有4个RcGeBP基因不均匀分布于3条染色体上且RcGeBP基因编码的蛋白质均为亲水性蛋白,高级结构以无规则卷曲为主。GeBP的进化树构建显示,蓖麻GeBP蛋白与番茄GeBP蛋白亲缘关系最近,蓖麻的GeBP基因分别与番茄和拟南芥的GeBP基因发生了3次和2次基因组间的复制。Ka/Ks结果显示,GeBP基因在物种间的进化是钝化的。启动子顺式作用元件预测结果显示,RcGeBP启动子区域含多个逆境响应和激素诱导类元件。RcGeBP基因的FPKM表达值显示,这些基因拥有组织表达特性且表达受到干旱和盐胁迫的激活。RcGeBP2蛋白质亚细胞定位结果显示,其定位在细胞核。综上,该研究对探明蓖麻RcGeBP基因家族参与盐和干旱胁迫的交叉调控具有一定的参考价值。
The GeBP gene family encodes a typical transcription factor that affects plant development and enhances plant survival in adverse environments by regulating the growth and differentiation of epidermal hairs.In order to explore the distribution of GeBP gene in the castor genome and the function of the characteristic gene RcGeBP 2,all the GeBP genes of castor were identified in this paper.The RcGeBP 2 gene was cloned by RT-PCR and its molecular characteristics were analyzed,including bioinformatics,subcellular localization and expression pattern.The results showed that four RcGeBP genes were unevenly distributed on three chromosomes in the genome of castor.The proteins encoded by RcGeBP genes were hydrophilic proteins,and the advanced structure was mainly random coil.The phylogenetic tree construction of GeBP showed that the GeBP protein of castor had the closest relationship with the GeBP protein of tomato.The GeBP gene of castor had three and two genome duplications with the GeBP gene of tomato and Arabidopsis thaliana,respectively.The results of Ka/Ks showed that the evolution of GeBP gene among species was passivated.The results of cis-acting element prediction showed that the RcGeBP promoter region contained multiple stress response and hormone-inducible elements.The FPKM expression value of RcGeBP genes showed that these genes had tissue expression characteristics and their expression was activated by drought and salt stress.The subcellular localization of RcGeBP2 protein showed that it was localized in the nucleus.In summary,this study has certain reference value for exploring the cross-regulation of castor RcGeBP gene family involved in salt and drought stress.
作者
朱贵爽
李艳肖
张安宁
孙浩楠
徐兴源
李志刚
向殿军
ZHU Guishuang;LI Yanxiao;ZHANG Anning;SUN Haonan;XU Xingyuan;LI Zhigang;XIANG Dianjun(College of Agriculture,Inner Mongolia Minzu University,Tongliao 028000,Inner Mongolia,China)
出处
《浙江农业学报》
CSCD
北大核心
2024年第8期1731-1740,共10页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(32060492)
内蒙古自治区自然科学基金(2022MS03057,2023MS03032)。
关键词
蓖麻
GeBP
基因克隆
表达
亚细胞定位
castor
GeBP
gene cloning
expression
subcellular localization
