摘要
目的制备双模态碳量子点(carbon dots,CDs)探针并探索其示踪间充质干细胞(MSCs)的可行性。方法2022年10月至2023年5月,以钆喷酸葡胺、柠檬酸及二乙烯三胺为前驱体,采用水热法合成Gd∶CDs[钆(Gd)掺杂的碳量子点],进行表征并用于MSCs标记及成像。检测不同浓度Gd∶CDs(10、25、50 nmol/L)对MSCs成骨分化及成脂肪分化的影响。结果Gd∶CDs生物安全性好,荧光量子产率为76%,纵向弛豫率为5.5727 mmol^(-1)·s^(-1),具备双模态成像潜力,可用于MSCs标记。不同浓度Gd∶CDs(10、25、50 nmol/L)标记的MSCs经成骨诱导后,吸光度[D(λ)]分别为2.368±0.014、2.253±0.084及2.133±0.127,与对照组D(λ)(2.334±0.062)相比,高浓度Gd∶CDs对MSCs成骨分化有少许影响(P<0.05),中低浓度的Gd∶CDs标记对MSCs成骨分化无明显影响(P>0.05)。不同浓度Gd∶CDs(10、25、50 nmol/L)标记的MSCs经成脂肪诱导后,D(λ)分别为0.274±0.036、0.286±0.032、0.262±0.065,与对照组D(λ)(0.254±0.026)相比,各组对MSCs的成脂肪分化无明显影响(P>0.05)。结论采用水热法制备的Gd∶CDs荧光量子产率及纵向弛豫率高,具有示踪MSCs的潜能,且对MSCs的分化影响较小。
Objective To prepare bimodal carbon dot probes and explore their feasibility for tracking mesenchymal stem cells(MSCs).Methods Gadolinium-doped carbon dots(Gd∶CDs)were synthesized using Gd-DTPA,citric acid,and diethylenetriamine as precursors via a hydrothermal method from October 2022 to May 2023.The synthesized Gd∶CDs were characterized and used for labeling and tracing MSCs.The effects of different concentrations of Gd∶CDs(10,25,50 nmol/L)on the osteogenic and adipogenic differentiation of MSCs were assessed.Results Gd∶CDs showed good biocompatibility,with a fluorescence quantum yield of 76%and a longitudinal relaxivity of 5.5727 mmol^(-1)·s^(-1),indicating their potential for bimodal imaging and MSCs labeling.After osteogenic induction of MSCs labeled with different concentrations of Gd∶CDs(10,25,50 nmol/L),the optical density values were 2.368±0.014,2.253±0.084,and 2.133±0.127,respectively.Compared with the control group(optical density value of 2.334±0.062),the high concentration of Gd∶CDs had a slight effect on the osteogenic differentiation of MSCs(P<0.05),while the medium and low concentration groups showed no significant effect on osteogenic differentiation of MSCs(P>0.05).After adipogenic induction of MSCs labeled with different concentrations of Gd∶CDs(10,25,50 nmol/L),the optical density values were 0.274±0.036,0.286±0.032,and 0.262±0.065,respectively.Compared with the control group(optical density value of 0.254±0.026),none of the experimental groups showed a significant effect on the adipogenic differentiation of MSCs(P>0.05).Conclusion The Gd∶CDs synthesized in this experiment exhibits high fluorescence quantum yield and longitudinal relaxivity,making them suitable for tracking MSCs with minimal influence on their differentiation.
作者
袁军
常时新
王乐
周自明
YUAN Jun;CHANG Shixin;WANG Le;ZHOU Ziming(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Academy for Engineering and Technology,Fudan University,Shanghai 200433,China)
出处
《安徽医药》
CAS
2024年第10期2094-2098,I0008,共6页
Anhui Medical and Pharmaceutical Journal
