摘要
目的通过对不同等级白术饮片进行研究,完善白术质量评价方法,为传统白术饮片等级划分提供理论依据。方法根据传统分级依据,将白术饮片分为3个等级。采用HPLC及UV法,测定36批白术饮片不同等级中白术内酯Ⅰ、Ⅱ、Ⅲ及总多糖的含量,结合2020版《中国药典》,将白术不同等级饮片的外观性状与内在质控指标进行相关性分析。结果根据外观性状及多个质控指标的含量测定,通过主成分分析、聚类分析等,白术一级饮片质量明显优于二级、三级饮片(P<0.05),且二级、三级饮片之间无显著性差异(P>0.05),表明白术饮片可分为2个等级,不同等级饮片与白术内酯及总多糖含量高低呈正相关。结论基于多个质控指标结合外观性状的白术饮片等级划分,可为该饮片质量控制与等级研究提供一定科学依据。
Objective Through the study of different grades for Atractylodis macrocephalae Rhizoma decoction pieces,improve the quality evaluation method,and provide theoretical basis for the classification of Atractylodis macrocephalae Rhizoma.Methods According to the traditional classification,the Atractylodis macrocephalae Rhizoma decoction pieces was divided into three grades.By HPLC and UV method,the determination of atractylodes lactoneⅠ,Ⅱ,Ⅲand the content of total polysaccharide for 36 batches of different grades of Atractylodis macrocephalae Rhizoma decoction pieces.According to the 2020 edition of Chinese pharmacopoeia,the correlation between the appearance traits of different grades of Atractylodis macrocephalae Rhizoma decoction pieces and the internal quality control indexes was analyzed.Results According to the appearance characters,multiple quality control index of content determination,through the principal component analysis,cluster analysis and so forth.The quality of first-grade decoction pieces of Atractylodis macrocephalae Rhizoma is significantly better than the second-grade and third-grade decoction pieces(P<0.05),and there is no significant difference between second-grade and third-grade decoction pieces(P>0.05).As a result,the Atractylodis macrocephalae Rhizoma decoction pieces can be divided into two grades,different grades with the content of atractylodes lactone and total polysaccharide were positively correlated.Conclusion The classification of Atractylodis macrocephalae Rhizoma decoction pieces based on multiple quality control indexes combined with appearance characters can provide a scientific basis for the quality control and grade study of this decoction piece.
作者
杜洪志
郑英
陈志琳
张敏杰
李玮
DU Hongzhi;ZHENG Ying;CHEN Zhilin;ZHANG Minjie;LI Wei(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Suiyang Hospital of Traditional Chinese Medicine,Suiyang 563300,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2024年第6期1537-1545,共9页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
贵州省科学技术厅重大专项课题(黔科合重大专项字[2015]6009-4号):贵州省15种重点发展药材的加工炮制技术集成及产业化应用,负责人:李玮
国家中医药管理局2015年中医药部门公共卫生专项(国家中医药科技[(2015)86号]):贵州省中药炮制技术传承基地,负责人:李玮
贵州省中医药管理局2019年度科学技术研究重点项目(QZYYZD-2019-01):贵州省中药(民族药)产地加工与饮片炮制信息数据库的开发与应用研究,负责人:杜洪志。
关键词
白术饮片
白术内酯
总多糖
外观性状
等级研究
Atractylodis macrocephalae Rhizoma decoction pieces
Atractylodes lactone
Total polysaccharide
Appearance characteristics
Grade study
