摘要
目的:探究宫颈癌组织中黏蛋白5B(MUC5B)表达及与人乳头状瘤病毒(HPV)E6/E7 mRNA表达关系。方法:回顾性收集2018年6月-2021年6月本院收治的疑似宫颈癌患者212例临床资料,均接受宫颈活检,根据病理学诊断结果分为宫颈上皮内瘤变(CIN)Ⅰ级组49例、Ⅱ级组50例、Ⅲ级组51例、宫颈癌组62例。采用全自动核酸检测系统检测宫颈组织HPV E6/E7 mRNA表达,免疫组化法测定宫颈组织MUC5B表达情况。分析宫颈癌组织MUC5B水平与患者临床病理特征关系;Spearman法分析分析MUC5B与HPV E6/E7 mRNA的相关性。采用Kaplan-Meier法分析宫颈癌组织MUC5B表达与患者生存关系。结果:MUC5B阳性表达率、HPV E6/E7 mRNA阳性表达率在CINⅠ级组(24.5%、32.7%)、CINⅡ级组(46.0%、54.0%)、CINⅢ级组(66.7%、74.5%)、宫颈癌组(77.4%、82.3%)均依次增加;不同MUC5B蛋白表达患者其淋巴结转移、浸润深度存在差异;MUC5B与HPV E6/E7 mRNA表达呈正相关;Kaplan-Meier生存曲线显示,MUC5B阴性组3年累积生存率(86.9%)高于MUC5B阳性组(71.7%)(均P<0.05)。结论:宫颈癌组织中MUC5B高表达,且MUC5B蛋白表达与HPV E6/E7 mRNA表达呈正相关,且与患者预后有关。
Objective:To investigate the expression of the mucin 5B(MUC5B)level in cervical cancer tissue,and to study its correlation with the E6/E7 mRNA expression of human papilloma virus(HPV).Methods:The clinical data of 212 patients with suspected cervical cancer who had admitted in hospital from June 2018 to June 2021 were collected retrospectively.All these patients had undergone cervical biopsy and were divided into group A(49 cases with cervical intraepithelial neoplasia(CIN)I),group B(50 cases with CIN II),group C(51 cases with CIN III)and group D(62 cases with cervical cancer)according to the pathological results of the patients.The fully automated nucleic acid detection system was used to detect the HPV E6/E7 mRNA expression,and the immunohistochemical method was used to determine the expression of MUC5B in the different cervical cancer tissues.The correlation between the level of MUC5B in the cervical cancer tissue of the patients and their clinicopathological characteristics was analyzed.Spearman method was used to analyze the correlation between the MUC5B level of the patients and their HPV E6/E7 mRNA expression.Kaplan-Meier method was used to analyze the correlation between the expression of MUC5B in cervical cancer tissues of the patients and their survival rate.Results:The positive expression rates of MUC5B and HPV E6/E7 mRNA of the patients in group A(24.5%and 32.7%),in group B(46.0%and 54.0%),in group C(66.7%and 74.5%)and in group D(77.4%and 82.3%)had increased gradually.The patients with different MUC5B protein expression had the different lymph node metastasis and the different invasion depth of the cancer.The MUC5B expression of the patients with cervical cancer was positively correlated with their HPV E6/E7mRNA expression.Kaplan Meier survival curve showed that the cumulative survival rate(86.9%)of the patients with negative MUC5Bin 3years was significantly higher than that(71.7%)of the patients with positive MUC5B(all P<0.05).Conclusion:MUC5Bis highly expressed in cervical cancer tissues of the patie
作者
马菁雯
张琰
MA Jingwen;ZHANG Yan(Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai,201900)
出处
《中国计划生育学杂志》
2024年第9期2132-2135,2140,F0003,共6页
Chinese Journal of Family Planning
