摘要
本试验旨在研究甘薯和芋头在闽东山羊瘤胃中的降解特性及表面附着微生物群落动态变化。选用4头14月龄健康装有永久瘤胃瘘管的闽东公山羊[平均体重(26.60±2.35)kg],采用尼龙袋法分别在2、4、8、16、24、36和48 h测定甘薯和芋头的营养成分的动态降解率及降解过程中表面附着微生物变化。结果表明:1)甘薯和芋头的瘤胃降解率随滞留时间的延长均逐渐升高。干物质(DM)有效降解率分别为66.48%和62.88%,粗蛋白(CP)有效降解率分别为34.28%和32.62%,淀粉有效降解率分别为53.44%和43.60%,其中甘薯淀粉有效降解率显著高于芋头(P<0.05)。2)alpha及beta多样性结果表明,甘薯和芋头在不同滞留时间点对瘤胃细菌的丰富度、多样性和组成无显著影响(P>0.05)。门水平上,两组间优势菌均为拟杆菌门和厚壁菌门。属水平上,两组间优势菌属均为普雷沃氏菌属。Mantel检验进一步表明,假丁酸弧菌属与甘薯和芋头瘤胃降解率均呈显著正相关(r>0,P<0.05)。3)功能预测分析表明,甘薯和芋头通过磷酸戊糖途径、乙醛酸和二羧酸代谢以及丙酸代谢调控瘤胃微生物相互作用利用淀粉的功能。综上所述,甘薯和芋头具有较好的瘤胃降解性能,其中甘薯DM和淀粉降解率更佳。在降解过程中,两种饲料均不会破坏瘤胃微生物的动态平衡,因此甘薯和芋头是具有潜力的精饲料资源。
The aim of this experiment was to investigate the degradation characteristics and dynamic changes in surface attached microbial communities of sweet potato(Ipomoea batatas)and taro(Colocasia esculenta)in the rumen of Mindong goats.Four healthy male Mindong goats,aged 14 months with permanent rumen fistulas,were selected(average weight 26.60±2.35 kg).The dynamic degradation rate of nutritional components in sweet potato and taro were measured using a nylon bag method at 2,4,8,16,24,36,and 48 hours,during the degradation process.Surface attached microbial changes were also measured at these same time steps.It was found that:1)The rumen degradation rates of sweet potato and taro gradually increased with increasing degradation time.The effective degradation rates of dry matter(DM)were 66.48%and 62.88%for sweet potato and taro,respectively,while the effective degradation rates of crude protein(CP)were 34.28%and 32.62%,and the effective degradation rates of starch were 53.44%and 43.60%.Notably,the effective degradation rate of sweet potato starch was significantly higher than that of taro(P<0.05).2)The alpha and beta diversity results showed that sweet potato and taro had no significant effect on the richness,diversity,and composition of rumen bacteria at different degradation time points(P>0.05).Bacteroidetes was identified as the predominant bacterial phylum in the rumen microbial communities for both sweet potato and taro groups.At the genus level,the dominant bacterial genus for both groups was Prevotella.A Mantel test further indicated that Pseudobutyrivibrio was significantly positively correlated with rumen degradation in both sweet potato and taro(r>0,P<0.05).3)Functional prediction analysis showed that sweet potato and taro regulated the interaction of rumen microorganisms to utilize starch through the pentose phosphate pathway,through glyoxylate and dicarboxylate metabolism and through propanoate metabolism.In conclusion,both sweet potato and taro exhibit favourable rumen degradation performance,but the degr
作者
顾明明
姜幸慧
马志毅
邱水玲
刘浩宇
张洺瑞
卢佳宁
丘宇俊
王本治
甘乾福
GU Ming-ming;JIANG Xing-hui;MA Zhi-yi;QIU Shui-ling;LIU Hao-yu;ZHANG Ming-rui;LU Jia-ning;QIU Yu-jun;WANG Ben-zhi;GAN Qian-fu(College of Animal Science(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《草业学报》
CSCD
北大核心
2024年第9期169-184,共16页
Acta Prataculturae Sinica
基金
福建省科技计划项目(高校产学合作项目,2023N5004,星火项目,2023S0007,2023S0015和2023S0054,对外合作项目2023I1009)
福建农林大学农村振兴服务团队-草食性动物产业服务团队(11899170139)
福建农林大学科技创新专项基金(CXZX2020057A)资助。
关键词
甘薯
芋头
闽东山羊
瘤胃降解率
瘤胃微生物
sweet potato
taro
Mindong goat
rumen degradation rate
rumen microorganisms
