摘要
旨在研究布氏乳杆菌和不同糖类联用对紫花苜蓿青贮营养成分、发酵品质、瘤胃降解率以及有氧稳定性的影响。以紫花苜蓿为原料,采用了4种处理:LB(添加1×10^(6)cfu·g^(-1)布氏乳杆菌);GLB(添加1×10^(6)cfu·g^(-1)布氏乳杆菌和2%葡萄糖);FLB(添加1×10^(6)cfu·g^(-1)布氏乳杆菌和2%岩藻多糖);CK(无添加)。青贮60 d后,测定以上4个处理紫花苜蓿的营养成分、发酵品质、瘤胃降解率和有氧稳定性。结果表明,在营养成分上,与CK相比各处理均显著提高了紫花苜蓿青贮料的干物质(DM)、粗蛋白(CP)和可溶性碳水化合物(WSC)含量(P<0.05),其中GLB处理DM和CP含量最高,FLB处理WSC含量最高。各处理均能显著降低酸性洗涤纤维(ADF)含量(P<0.05),GLB处理能显著降低青贮料中性洗涤纤维(NDF)含量(P<0.05)。发酵品质上,除FLB处理外其余处理均能显著降低青贮料pH(P<0.05),GLB处理显著增加了乳酸(LA)和丙酸(PA)含量(P<0.05),显著降低了氨态氮/总氮(AN/TN, P<0.05)。LB和FLB处理LA、乙酸(AA)含量和AN/TN显著降低(P<0.05),PA含量显著增加(P<0.05)。在瘤胃降解方面,除GLB处理的ADF有效降解率无显著提高外(P>0.05),各处理DM、CP、ADF和NDF有效降解率均显著提升(P<0.05)。与CK相比,各处理均能在有氧暴露6 d显著降低pH、酵母菌和好氧菌数(P<0.05),其中FLB处理表现最佳。根据隶属函数法综合评价各项指标,各处理按优劣排序为:GLB(0.76)>LB(0.53)>FLB(0.52)>CK(0.14)。综上所述,LB、GLB和FLB处理均对紫花苜蓿青贮营养成分、发酵品质、瘤胃降解率及有氧稳定性有积极影响,其中GLB处理效果最好。
The aim of this experiment was to investigate the effects of Lentilactobacillus buchneri combined with different sugars on the nutrient composition,fermentation quality,rumen degradation rate and aerobic stability of alfalfa silage.Four treatments were employed:LB(1×10^(6) cfu·g^(−1) L.buchneri),GLB(1×10^(6) cfu·g^(−1) L.buchneri and 2% glucose),FLB(1×10^(6) cfu·g^(−1) L.buchneri and 2%fucoidan),CK(no additive).After 60 days of ensiling,the nutrient composition,fermentation quality,rumen degradation rate,and aerobic stability of alfalfa silages with the above four treatments were measured.It was found that in terms of nutrient composition,compared with the CK,all treatments significantly increased the alfalfa silage dry matter(DM),crude protein(CP)and water-soluble carbohydrate(WSC)contents(P<0.05)and the DM and CP contents were highest in the GLB treatment,while the WSC content was highest in the FLB treatment.Compared to CK,all treatments significantly reduced the content of acid detergent fiber(ADF)(P<0.05)and the GLB treatment significantly reduced the content of neutral detergent fiber(NDF,P<0.05).With respect to fermentation quality,the LB and GLB treatments significantly reduced the silage pH(P<0.05)compared with CK,while the GLB treatment significantly increased the content of lactic acid(LA)and propionic acid(PA)(P<0.05),and significantly reduced the ammonia nitrogen∶total nitrogen ratio(AN∶TN)(P<0.05).Compared to CK,the content of LA,acetic acid(AA)and AN∶TN in LB and FLB treatments decreased significantly(P<0.05),while PA increased significantly(P<0.05).In terms of rumen degradation,except GLB treatment,the effective degradation rate of ADF was not significantly improved(P>0.05),the effective degradation rates of DM,CP,ADF and NDF in other treatments were significantly increased(P<0.05).Compared to CK,the pH,the number of yeasts and aerobic bacteria in all treatments were significantly reduced after 6 days of aerobic exposure(P<0.05),and FLB treatment was the best.Using to the members
作者
凌文卿
张磊
李珏
冯启贤
李妍
周燚
刘一佳
阳伏林
周晶
LING Wen-qing;ZHANG Lei;LI Jue;FENG Qi-xian;LI Yan;ZHOU Yi;LIU Yi-jia;YANG Fu-lin;ZHOU Jing(College of Animal Sciences(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou 350002,China;China National Engineering Research Center of Juncao Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《草业学报》
CSCD
北大核心
2023年第7期122-134,共13页
Acta Prataculturae Sinica
基金
国家自然科学基金项目(42075116)
福建农林大学杰出青年科研人才计划项目(xjq201817)和福建农林大学动物科学学院引进人才科研启动经费项目(71201801601)资助。
关键词
布氏乳杆菌
紫花苜蓿
发酵品质
瘤胃降解率
有氧稳定性
Lentilactobacillus buchneri
alfalfa
fermentation quality
rumen degradation rate
aerobic stability
