摘要
为了解绿豆种质资源遗传信息丰富度、掌握材料间亲缘关系,加强绿豆育种进程以及为新品种鉴定提供依据。本试验利用37对SSR引物对19份绿豆材料进行遗传多样性分析。结果表明,34对引物可扩增出清晰条带,扩增条带总数为216个,其中多态性条带数为100个,平均多态率为46.30%。每对引物的平均有效等位基因为1.4971,Nei's基因多样性指数(H)均值为0.2908,平均Shannon信息指数(I)为0.4141。供试材料的遗传距离介于0.1831~0.5645之间,由此表明材料间遗传关系较近,遗传背景狭窄。以GD均值0.3389为基准,可将其分为4个类群,大部分生境相似的材料被聚为一类。试验还筛选出一对特异性引物bm487,可有效地鉴定材料间的差异。
The study was conducted to understand the genetic information richness of mung bean germplasm resources,to grasp the kinship between materials,to enhance the mung bean breeding process and to provide a basis for the identification of new varieties.In this work,37 SSR primer pairs were used to analyze the genetic diversity of 19 mung bean materials.The results showed that 34 primer pairs could amplify clear bands,and the total number of amplified bands was 216,among which the number of polymorphic bands was 100,and the average polymorphism rate was 46.30%.The average effective allele of each primer pair was 1.4971,the mean value of Nei's genetic diversity index(H)was 0.2908,and the mean Shannon information index(H)was 0.4141.The genetic distances of the test materials ranged from 0.1831 to 0.5645,thus indicating that the materials were genetically close to each other and the genetic background was narrow.With the GD mean value of 0.3389,the test materials could be divided into four taxa,and most of the materials with similar habitats were clustered into one group.The experiment also screened a pair of specific primers bm487,which can effectively identify the differences among the materials.
作者
何瑞超
李小雷
贾学宇
王雪
吴小燕
鲍红春
He Ruichaol;Li Xiaolei;Jia Xueyul;Wang Xue;Wu Xiaoyan;Bao Hongchun(College of Agriculture,Inner Mongolia Agricultural University,Hohhot,010019;Inner Mongolia Autonomous Region Horticultural Research Institute,Hohhot,010010)
出处
《分子植物育种》
CAS
北大核心
2024年第17期5640-5647,共8页
Molecular Plant Breeding
基金
内蒙古农业大学博士科研启动基金项目(BJ08-8)
国家自然科学基金项目(31302021)
内蒙古自然科学基金项目(2010MS0502)共同资助。
关键词
SSR
遗传多样性
绿豆
聚类分析
种质资源
SSR
Genetic diversity
Vigna radiata
Clustering analysis
Germplasm resources
