摘要
【目的】为明确江西省烟草赤星病菌Alternaria alternat的遗传多样性,开展了相关研究。【方法】运用RAPD分子标记法对江西省6个主要烟区赤星病分离出的37个菌株进行了遗传多样性、遗传结构与分化及种间遗传变异分析。【结果】其中6个不同地理种群的Nei’s基因多样性指数(He)为0.1618~0.3209,Shannon信息指数(I)为0.2419~0.4785,表明不同地理来源种群之间的链格孢菌具有丰富的遗传多样性;遗传距离分析表明,兴国和信丰的菌株遗传距离最小,亲缘关系较近,石城和黎川的菌株遗传距离最大,亲缘关系较远;居群每代迁移数(Nm)为1.8171,表明不同地理来源的种群间存在一定程度的基因流动;利用NTSYS软件按UPGMA法聚类分析,当相似系数划定为0.56时,可将37个菌株划分为10个类群,37个菌株基本上以地理来源聚类到不同分支,表明菌株间的遗传多样性受其地理来源影响明显。【结论】利用RAPD分子标记法明确了江西省主要烟区烟草赤星病菌Alternaria alternat的遗传多样性,为江西省烟区选育抗赤星病品种及其综合防治提供措施奠定理论基础。
[Objective]To clarify the genetic diversity of Alternaria alternata in Jiangxi Province,relevant studies were conducted.[Method]Random Amplified Polymorphic DNA(RAPD)molecular markers were used to analyze 37 isolates of Alternaria alternata from 6 major tobacco-producing areas in Jiangxi Province.[Result]Nei’s gene diversity index(He)of 6 different geographical populations was 0.1618~0.3209,and Shannon information index(I)was 0.2419~0.4785.A.alternata from different geographical sources had rich genetic diversity,and genetic distance analysis showed that strains from Xingguo and Xinfeng counties had the smallest genetic distance and close genetic relationship,while that form Shicheng and Lichuan had the largest genetic distance and far genetic relationship.The migration number per generation(Nm)of the population was 1.8171,indicating that there was a certain degree of gene flow among populations from different geographical origins.Using NTSYS software and UPGMA method for cluster analysis,when the similarity coefficient was defined as 0.56,37 strains could be divided into 10 groups,and 37 strains were basically clustered into the corresponding branches by geographical origin,indicating that there was obvious correlation between genetic diversity among strains and geographical origin.[Conclusion]The genetic diversity of A.alternate isolated from 6 tobacco-producing areas in Jiangxi Province was confirmed by RAPD,making the basis for the tobacco varieties against A.alternate as well as the disease control.
作者
张文梅
蔡梁
李松宴
胡长志
崔汝强
ZHANG Wenmei;CAI Liang;LI Songyan;HU Changzhi;CUI Ruqiang(Guangchang Branch of Fuzhou Tobacco Company,Guangchang,Jiangxi 344000,China;School of Agronomy Science,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《生物灾害科学》
2024年第3期399-406,共8页
Biological Disaster Science
