摘要
SSR标记是一种基于DNA长度多态性的分子标记技术,是进行群体遗传结构分析、构建遗传连锁图谱非常有效的工具。由于SSR标记是特异引物标记,必须在知道某个物种DNA序列的前提下,才能设计引物进行PCR扩增,故而存在一个引物开发的问题。从SSR标记的发展历程来看,开发SSR引物的方法有经典的构建与筛选基因组文库的方法、微卫星富集法、省略筛库法和数据库搜索法等四种。综述了这4种方法的操作流程及其在实际应用中的优缺点,并对近年来SSR引物在相近的物种间转移使用的情况作了介绍。
SSRs is one of molecular markers technology based on DNA length polymorphism and an efficient tool for population genetic studies and primary genetic linkage maps construction. Because of a special primer marker,It's necessary to know a species DNA sequence in order to design primers for PCR testing. That is to say,there is a problem of SSR primer development. For the progress of SSR marker technology,the methods of developing SSR primer could be divided into four kinds: traditional constructing and screening genome library procedure,the SSR richment procedure,avoiding screening genome library procedure and database search procedure. This paper reviewed these four methods'operation processes and their advantages and disadvantages. In addition,transferability of SSR primers in closely related species were introduced in recent years.
出处
《遗传》
CAS
CSCD
北大核心
2004年第5期769-776,共8页
Hereditas（Beijing)
