摘要
目的 研究中药合剂Zgy-2对高糖环境下血管内皮细胞的影响。方法 培养脐静脉血管内皮细胞,取生长良好的2~5代细胞分为正常对照组、高糖培养组、高糖培养+低剂量药物组、高糖培养+高剂量药物组。采用MTT法、细胞计数法测定细胞增殖和活力,采用免疫荧光染色法检测血管内皮生长因子(VEGF)的表达,采用流式细胞仪检测细胞凋亡率。结果 与正常对照组比较,高糖培养组细胞增殖活力OD值和细胞数目均明显降低,VEGF表达减少,细胞凋亡率升高(P<0.05);与高糖培养组比较,高糖培养+高剂量药物组和高糖培养+低剂量药物组细胞增殖活力OD值和细胞数目均明显升高,VEGF表达升高,细胞凋亡率降低(P<0.05)。结论 中药合剂Zgy-2可促进高糖环境下血管内皮细胞的增生和迁移,增强VEGF的表达,抑制高糖环境下血管内皮细胞的凋亡。
Objective To study the effect of Chinese medicine mixture Zgy-2 on vascular endothelial cells in high glucose environment.Methods The umbilical vein endothelial cells were cultured,and the 2-5 generations of well-grown cells were divided into normal control group,high glucose culture group,high glucose culture+low dose drug group,and high glucose culture+high dose drug group.The proliferation and viability of the cells were measured by MTT method and cell counting method.The expression of vascular endothelial growth factor(VEGF) was detected by immunofluorescence staining.The apoptosis rate was detected by flow cytometry.Results Compared with normal control group,OD value and number of cells in high glucose culture group were significantly decreased,VEGF expression was decreased,and apoptosis rate was increased(P<0.05).Compared with high glucose culture group,OD value and number of cell in high glucose culture+high dose drug group and high glucose culture+low dose drug group were significantly increased,VEGF expression were increased,and apoptosis rate were decreased(P<0.05).Conclusion Chinese medicine mixture Zgy-2 can promote the proliferation and migration of vascular endothelial cells,enhance the expression of VEGF,and inhibit the apoptosis of vascular endothelial cells under high glucose environment.
作者
杜艳平
孙永琨
DU Yanping;SUN Yongkun(Xinxiang Medical University,Henan Province,Xinxiang 453003,China;不详)
出处
《临床合理用药杂志》
2024年第25期27-30,40,共5页
Chinese Journal of Clinical Rational Drug Use
基金
河南省高等学校实验室工作研究会项目(ULAHN202367)。
关键词
中药合剂
Zgy-2
内皮细胞
高糖环境
血管内皮生长因子
Chinese medicine mixture
Zgy-2
Endothelial cells
High glucose environment
Vascular endothelial growth factor
