摘要
目的:探讨糖尿病前期痰证患者的肠道菌群及血清代谢产物的差异性表达与潜在生物标志物。方法:共纳入34例糖尿病前期痰证患者为痰证组,并选取同期体检的37例健康人为正常组,留取2组研究对象的血清和粪便标本,采用液相色谱-质谱法(LC-MS)非靶向代谢组学及16S rRNA高通量测序技术,来检测两者的血清代谢物和差异肠道菌群,探讨糖尿病前期痰证与血清代谢物、肠道菌群之间的关联性。结果:痰证组证素分布情况中,病性证素按照频数和占比前5位兼杂证素为湿(73.53%)、气滞(58.82%)、阴虚(50.00%)、血瘀(41.18%)、热(35.29%);病位证素按照频数和占比前3位主要病位证素为脾(100.00%)、肝(41.18%)、肾(23.53%)。16S rRNA高通量测序结果显示,两组有44种差异肠道菌群,在目属上,双歧杆菌、韦荣氏球菌、罗氏菌两组间差异具有统计学意义(P<0.05)。痰证组在肠道菌群多样性、丰富度和均匀度较正常组减少,但差异无统计学意义,群落结构上两组亦差异无统计学意义。血清代谢组学结果显示,两组共同拥有13种差异代谢物,主要富集在氨基酸代谢、胆汁分泌和胆汁酸生物合成、脂质代谢等通路(P<0.05)。将差异代谢物、肠道菌群和证素进行相关性分析,(1)赖氨酸和脾、阴虚、血瘀呈正相关;牛磺胆酸与肝、肾、血瘀、湿呈正相关,与阴虚、热呈正相关;牛磺鹅去氧胆酸与肝、湿呈正相关;花生四烯酸与湿呈负相关;葡萄糖与脾、血瘀呈正相关。(2)梭杆菌属和脾、肾、阴虚、气滞呈正相关;罗氏菌属与脾呈负相关;萨特氏菌属与湿呈负相关;拟杆菌属与脾、肾呈负相关;双歧杆菌与湿、脾呈负相关。(3)双歧杆菌与甘氨酸、苏氨酸、赖氨酸、鹅去氧胆酸呈正相关,与胆酸呈负相关,与牛磺鹅去氧胆酸、丙酮酸呈正相关;梭杆菌属与甘氨酸呈正相关,与苏氨酸、赖氨酸呈正相关;毛螺菌属与甘氨酸、苏�
Objective to investigate the differential expression of intestinal flora and serum metabolites and potential biomarkers in patients with pre-diabetic sputum syndrome.Method A total of 34 patients with pre-diabetic sputum syndrome were included as the phlegm syndrome group,and 37 healthy people were selected as the normal group.Serum and fecal samples of the two groups were collected,and liquid chromatography-mass spectrometry(LC-MS)non-targeted metabolomics and 16S rRNA high-throughput sequencing technology were used to detect serum metabolites and different intestinal flora of the two groups and explore the relationship among pre-diabetic sputum syndrome,serum metabolites,and intestinal flora.Result In the distribution of disease syndrome elements in the phlegm syndrome group,the first five disease syndrome elements in terms of frequency and proportion were dampness(73.53%),Qi stagnation(58.82%),Yin deficiency(50.00%),blood stasis(41.18%),and heat(35.29%).According to the frequency and proportion of disease location syndrome elements,the first three main disease location syndrome elements were spleen(100.00%),liver(41.18%),and kidney(23.53%).The results of 16S rRNA high-throughput sequencing showed that there were 44 different intestinal flora between the two groups.In order genus,there were significant differences in Bifidobacterium,Veillonococcus,and Roseococcus between the two groups(P<0.05).The diversity,abundance,and evenness of intestinal flora in the phlegm syndrome group were lower than those in the normal group,with the difference not statistically significant.There was no significant difference in the community structure between two groups.The results of serum metabolomics showed that there were 13 differential metabolites in the two groups,which were mainly concentrated in amino acid metabolism,bile secretion,bile acid biosynthesis,and lipid metabolism(P<0.05).The correlation among differential metabolites,intestinal flora,and syndrome elements was analyzed,and the results showed that①lysine was pos
作者
王志汕
黄亮亮
张玮
陈明燚
肖绍坚
陈淑娇
WANG Zhishan;HUANG Liangliang;ZHANG Wei;CHEN Mingyi;XIAO Shaojian;CHEN Shujiao(The Third People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine(TCM),Fuzhou 350122,China;School of Chinese Medicine,Fujian University of TCM,Fuzhou 350122,China;The People's Hospital Affiliated to Fujian University of TCM,Fuzhou 350000,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2024年第18期146-156,共11页
Chinese Journal of Experimental Traditional Medical Formulae
基金
第五批全国中医临床优秀人才研修项目(国中医药人教函[2022]1号)
福建省自然科学基金计划项目(2020J01766)。
关键词
糖尿病前期
痰证
肠道菌群
代谢组学
pre-diabetes
phlegm syndrome
intestinal flora
metabonomics
