摘要
目的基于腹腔注射聚肌苷酸-聚胞苷酸(PolyI:C)建立小鼠胸腺萎缩模型,比较三叶青石油醚提取物(petroleum ether extract,PEE)、乙酸乙酯提取物(ethyl acetate extract,EAE)、正丁醇提取物(n-butanol extract,BE)和水提取物(water extract,WE)抗胸腺萎缩的药效作用,初步探讨其作用机制。方法100只ICR小鼠随机分为空白对照组、模型组和4种提取物的低、高剂量组,共10组,每组10只。空白对照组、模型组同时给予同体积的纯化水,连续灌胃10 d。给药第9天开始,除空白对照组腹腔注射生理盐水外,其余组别均腹腔注射16 mg·kg^(-1)的PolyI:C溶液,连续2 d。取胸腺称重并计算脏器指数,取血进行白细胞分析及IL-2、TNF-α的检测,分析胸腺组织的苏木精-伊红染色结果,通过Western blotting检测TNF-α、p-NF-κB、NF-κB的表达。结果相较于模型组,提取物各组均可不同程度提升胸腺质量和胸腺指数,及皮质和髓质面积比值和髓质1 mm~2的胸腺小体数量,显著降低血浆中TNF-α水平,明显抑制NF-κB的磷酸化,降低TNF-α的蛋白表达,且在不同指标上的作用强度各有优劣。WE低剂量组、EAE低剂量组和PEE高剂量组外周血白细胞数、淋巴细胞绝对值和淋巴细胞百分率升高;WE低剂量组和BE高剂量组IL-2水平显著升高,这些作用表现尤为突出。结论三叶青块根4种提取物可不同程度改善胸腺萎缩和胸腺组织形态改变,调节造模后动物外周血白细胞失衡,可能与抑制PolyI:C诱导转录因子NF-κB的磷酸化、降低促炎因子的蛋白表达有关。
OBJECTIVE To compare the anti-thymic atrophy effects of petroleum ether(PEE),ethyl acetate(EAE),n-butanol(BE)and water extract(WE)fractions from root tubers of Tetrastigma hemsleyanum Diels et.Gilg based on mice thymic atrophy model established by intraperitoneal injection of PolyI:C to preliminarily investigate the mechanisms of its effects.METHODS One hunderd ICR mice were randomly divided into 10 groups:control group,model group,low-and high-dose groups of the four extract fractions,with 10 mice in each group.The control group and model group were given equal volume of purified water and gavaged for 10 d.Starting on the 9th day,PolyI:C solution of 16 mg·kg^(-1) was injected intraperitoneally for 2 consecutive days,except for the control group,which was injected intraperitoneally with physiological saline.Samples of thymics were weighed and and visceral index was calculated,blood samples were taken for analysis of IL-2,TNF-αand WBC,thymic histopathology was analyzed using hematoxylin-eosin staining,Western blotting was performed to detect the expression of TNF-α,p-NF-κB and NF-κB.RESULTS Compared with the model group,all groups of extracts could enhance the thymus weight and thymus index,as well as the cortex and medulla area ratio and the number of thymic corpuscles in 1 mm2 of medulla to different degrees,and at the same time,significantly reduce the level of TNF-αin plasma,evidently inhibited the phosphorylation of NF-κB,and reduced the protein expression of TNF-α,and each of them having distinct advantages and disadvantages.Peripheral blood white blood cell,lymphocyte absolute count and lymphocyte percentage were elevated in the low-dose WE group,low-dose EAE Chin J Mod Appl Pharm,2024 July,Vol.41,No.14 group and high dose PEE group,IL-2 levels were significantly elevated in the low-dose WE group and high dose BE group,and these effects were particularly well demonstrated.CONCLUSION Four extracts from root tubers of Tetrastigma Hemsleyanum Diels et.Gilg can improve morphological changes and atrophy
作者
谢珍
张晨辉
陆静娴
业康
何立成
陈舒怀
匡荣
张劲松
陈碧莲
XIE Zhena;ZHANG Chenhui;LU Jingxian;YE Kang;HE Licheng;CHEN Shuhuai;KUANG Rong;ZHANG Jinsong;CHEN Bilian(Zhejiang Institute for Food and Drug Control,NMPA Key Laboratory for Animal Alternative Testing Technology of Cosmetics,Hangzhou 310052,China;Zhejiang Institute for Food and Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Medicine(Traditional Chinese Patent Medicine),Hangzhou 310052,China;Zhejiang Research Institute of Traditional Chinese Medicine Co.,Ltd.,Hangzhou 310023,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2024年第14期1913-1920,共8页
Chinese Journal of Modern Applied Pharmacy
基金
浙江省食品药品检验研究院新型冠状病毒应急科研攻关专项(zsyjk2020001)。
关键词
三叶青块根
提取物
胸腺萎缩
root tubers of Tetrastigma Hemsleyanum Diels et.Gilg
extract
thymic atrophy
