摘要
目的基于肠道菌群探讨瘀血痹片对类风湿关节炎的作用。方法36只SD大鼠随机分为正常对照组、胶原诱导型类风湿关节炎(CIA)组、甲氨蝶呤0.002 g·kg^(-1)剂量组、瘀血痹片0.4、0.8、1.6 g·kg^(-1)(YXBL、YXBM、YXBH)剂量组,后5组采用牛Ⅱ型胶原乳剂诱导类风湿关节炎模型,除正常组和CIA组大鼠给予三蒸水外,其余各组按上述剂量灌胃给药。30 d后,测量各组大鼠脾脏、胸腺质量,计算脾脏、胸腺指数;HE染色观察滑膜组织、脾脏和结肠病理变化;16S rRNA高通量测序技术测定各组大鼠肠道菌群变化。结果瘀血痹片可使CIA大鼠脾脏指数、胸腺指数下降(P>0.05),足爪体积、踝周长、关节炎评分减少(P<0.01),滑膜组织增生减轻、炎性细胞浸润减少,改善脾脏和结肠病理变化,降低CIA大鼠肠道菌群中Fusicatenibacter、Erysipelotrichaceae_UCG-003、Monoglobus、UCG-005菌属的相对丰度(P<0.05),增加Prevotellaceae_UCG-003菌属的相对丰度(P<0.05)。结论瘀血痹片对类风湿关节炎具有改善作用,可能与其调控肠道菌群有关。
Objective To explore the effect of Yuxuebi tablet in the treatment of rheumatoid arthritis based on intestinal flora.Methods Thirty-six SD rats were randomly divided into normal control group,collagen induced rheumatoid arthritis(CIA)group,methotrexate 0.002 g·kg^(-1) dose group,Yuxuebi tablet 0.4,0.8,1.6 g·kg^(-1)(YXBL,YXBM,YXBH)dose groups,the latter five groups were treated with bovine typeⅡcollagen emulsion to induce rheumatoid arthritis model.Except for rats in the normal group and CIA group,which were given three distilled water,the other groups were given the above doses by intragastric administration.After 30 days,the weight of spleen and thymus of rats in each group was measured,and the spleen index and thymus index were calculated.HE staining was used to observe the pathological changes of synovial tissue,spleen and colon.16S rRNA high-throughput sequencing technology was used to detect the changes in intestinal flora of rats in each group.Results The spleen index and thymus index of CIA rats were reduced(P>0.05),the paw volume,ankle circumference and arthritis score were decreased(P<0.01),the synovial tissue hyperplasia and inflammatory cell infiltration were reduced,and the pathological changes of spleen and colon were improved after the CIA rats were treated with Yuxuebi tablet.Yuxuebi tablet could reduce the relative abundance of Fusicatenibacter,Erysipelotrichaceae_UCG-003,Monoglobus and UCG-005(P<0.05),and increase the relative abundance of Prevotellaceae_UCG-003 in the intestinal flora of CIA rats(P<0.05).Conclusion Yuxuebi tablet can improve rheumatoid arthritis,which may be related to its regulation of intestinal flora.
作者
邵平
韩凌
齐沐爽
任颖
朱婉君
代春美
胡相卡
SHAO Ping;HAN Ling;QI Mushuang;REN Ying;ZHU Wanjun;DAI Chunmei;HU Xiangka(Benxi National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines Co.,Ltd.,Benxi,Liaoning 117004,China;China Resources Liaoning Benxi Third Pharmaceutical Co.,Ltd.,Benxi,Liaoning 117004,China;Jinzhou Medical University,Jinzhou,Liaoning 121001,China)
出处
《石河子大学学报（自然科学版）》
CAS
北大核心
2024年第3期358-368,共11页
Journal of Shihezi University(Natural Science)
基金
辽宁省横向课题项目(2022-012)。
