摘要
该研究采用Illumina NovaSeq高通量测序技术分析两个贵州特色米酒产区-黔南州、黔东南州6个米酒曲样品的微生物群落多样性,并通过线性判别分析效应大小(LEfSe)对不同米酒曲中的差异菌群进行分析。结果表明,从6个米酒曲样品中共注释到18个细菌门、21个细菌属、14个真菌门和22个真菌属,6个米酒曲样品的共有优势细菌门(相对丰度>1%)为厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes),共有优势细菌属为葡萄球菌属(Staphylococcus);共有优势真菌门为毛霉菌门(Mucoromycota)和子囊菌门(Ascomycota),共有优势真菌属为根霉属(Rhizopus)。在门和属水平上,拟杆菌门、哈萨克斯坦酵母属(Kazachstania)、乳杆菌属(Lactobacillus)和伊萨酵母属(Issatchenkia)是引起黔南州和黔东南州米酒曲样品细菌和真菌类群差异最主要的菌群。该研究结果为进一步筛选功能微生物并制作强化酒曲、米酒发酵工艺控制奠定基础。
In this study,the microbial community diversity of 6 rice Jiuqu samples from two Guizhou characteristic rice wine producing areas-Qiannan and Qiandongnan was analyzed using Illumina NovaSeq high-throughput sequencing technology.The difference microbial community in different rice wine Jiuqu was analyzed by linear discriminant analysis effect size(LEfSe).The results showed that 18 bacterial phyla,21 bacterial genera,14 fungal phyla and 22 fungal genera were identified from 6 rice wine Jiuqu samples.The common dominant bacterial phyla(relative abundance>1%)of the 6 rice wine Jiuqu samples were Firmicutes,Proteobacteria and Bacteroidetes,and the common dominant bacterial genus was Staphylococcus.The common dominant fungal phyla were Mucoromycota and Ascomycota,and the common dominant fungal genus was Rhizopus.At phylum and genus level,Bacteroides,Kazachstania,Lactobacillus and Issatchenkia were the main bacteria groups causing the differences between the rice wine Jiuqu samples from Qiannan and Qiandongnan.The research laid a foundation for further screening functional microorganisms and controlling the fermentation process of fortified Jiuqu and rice wine.
作者
谢玲
龚小会
张东亚
陈晨
XIE Ling;GONG Xiaohui;ZHANG Dongya;CHEN Chen(Guizhou Light Industry Science Research Institute,Guiyang 550014,China;Guizhou Institute of Light Industry,Guiyang 550025,China)
出处
《中国酿造》
CAS
北大核心
2024年第8期170-176,共7页
China Brewing
基金
贵州省科技计划项目(黔科合服企[2021]3号)
贵州省科技计划项目(黔科合支撑[2021]一般103)
贵州科学院科研发展资金(黔科院科发[2023]02号)
贵州科学院省级科研专项资金项目(黔科院科专合字[2022]06号)
贵州科学院省级科研专项资金项目(黔科院科专合字[2021]01号)
贵州科学院省级科研专项资金项目(黔科院科专合字[2020]07号)
贵州科学院青年科学基金项目(黔科院J字[2023]01号)。
关键词
贵州特色米酒
酒曲
高通量测序
微生物群落
多样性
差异物种
Guizhou characteristic rice wine
Jiuqu
high-throughput sequencing
microbial community
diversity
differential species
