摘要
该研究以江苏丹阳某黄酒酒曲为原料,利用Illumina Miseq高通量测序技术对其微生物菌群多样性进行研究。结果表明,该酒曲中真菌菌群的多样性高于细菌,而丰富度低于细菌。从样品中共注释到7个细菌门,10个细菌属;6个真菌门,14个真菌属。优势细菌属(相对丰度≥1%)为乳杆菌属(Lactobacillus)、魏斯氏菌属(Weissella)、肠杆菌属(Enterobacter)、葡萄球菌属(Staphylococcus)、不动杆菌属(Acinetobacter)、泛菌属(Pantoea);优势真菌属(相对丰度≥1%)为曲霉属(Aspergillus)、短梗霉属(Aureobasidium)、芽枝霉属(Cladosporium)、威克汉姆酵母属(Wickerhamomyces)、青霉属(Penicillium)、木拉克酵母属(Mrakia)、根霉属(Rhizopus)、镰刀菌属(Fusarium)、帚枝霉属(Sarocladium)、篮状菌属(Talaromyces)。其中,短梗霉属(Aureobasidium)、篮状菌属(Talaromyces)均为酒曲微生物中报道较少的属,其特性和功能尚不清晰,有待进一步深入的研究。该研究结果为丹阳黄酒的发酵工艺改进和品质提升提供了理论依据。
In this study,the microbial community diversity in Huangjiu Jiuqu from Danyang,Jiangsu was studied by Illumina Miseq high-throughput sequencing technique.The results showed that the diversity of fungal community in the Jiuqu was higher than that of bacterial community,while the richness was lower than that of bacterial community.A total of 7 bacterial phyla,10 bacterial genera,and 6 fungal phyla,14 fungal genera were annotated from the sample.Lactobacillus,Weissella,Enterobacter,Staphylococcus,Acinetobacter and Pantoea were the dominant bacterial genera(relative abundance≥1%).Aspergillus,Aureobasidium,Cladosporium,Wickerhamyces,Penicillium,Mrakia,Rhizopus,Fusarium,Sarocladium and Talaromyces were the dominant fungal genera.Among the dominant fungal genera(relative abundance≥1%),Aureobasidium and Talaromyces were less reported in microbial genera of Jiuqu,their characteristics and functions were still not clear and need to be further studied.The results of the study provided a theoretical basis for improving the fermentation process and quality of Danyang Huangjiu.
作者
陈磊
戴亦军
CHEN Lei;DAI Yijun(Zhongbei College,Nanjing Normal University,Zhenjiang 212399,China;School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)
出处
《中国酿造》
CAS
北大核心
2024年第6期141-145,共5页
China Brewing
基金
南京师范大学中北学院青年教师培养计划项目(1677772002Y)。
关键词
黄酒酒曲
微生物菌群多样性
高通量测序
群落结构
Huangjiu Jiuqu
microbial community diversity
high-throughput sequencing
community structure
