摘要
目的 构建溃疡性结肠炎(UC)小鼠模型,探讨ε-聚赖氨酸、果胶及二者混合物对UC小鼠肠黏膜屏障的影响及可能机制。方法 雄性BALB/c小鼠40只,随机分为对照组、UC组、ε-聚赖氨酸组、果胶组、ε-聚赖氨酸+果胶组各8只。所有小鼠给予灌胃预处理,对照组、UC组给予饮用水20 mL/(kg·d),ε-聚赖氨酸组给予ε-聚赖氨酸10 mg/(kg·d),果胶组给予果胶200 mg/(kg·d),ε-聚赖氨酸+果胶组给予ε-聚赖氨酸10 mg/(kg·d)和果胶200 mg/(kg·d),灌胃4周后,UC组、ε-聚赖氨酸组、果胶组、ε-聚赖氨酸+果胶组小鼠自由饮用质量分数3%硫酸葡聚糖溶液9 d构建UC模型,对照组自由饮用饮用水9 d。造模后评价5组小鼠疾病活动指数(DAI)评分,测量结肠长度,采用HE染色法观察结肠组织病理形态,采用ELISA法检测血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平,采用实时荧光定量PCR法检测结肠组织闭合蛋白(Occludin)、紧密连接蛋白-1(ZO-1)mRNA相对表达量,采用Western blot法检测结肠组织Toll样受体4(TLR4)、核因子-κB p65(NF-κB p65)、p-NF-κB p65蛋白相对表达量,并计算p-NF-κB p65/NF-κB p65。结果 (1)UC组[(6.75±1.91)分]、ε-聚赖氨酸组[(5.75±1.75)分]、果胶组[(3.00±0.76)分]、ε-聚赖氨酸+果胶组[(6.00±3.21)分]DAI评分均高于对照组(0分)(P<0.05);UC组[(6.96±0.78)cm]、ε-聚赖氨酸+果胶组[(6.79±0.73)cm]结肠长度均短于对照组[(8.10±0.55)cm](P<0.05),ε-聚赖氨酸组、果胶组与对照组比较差异均无统计学意义(P>0.05);UC组、ε-聚赖氨酸+果胶组DAI评分均高于果胶组(P<0.05),结肠长度均短于果胶组(P<0.05),ε-聚赖氨酸组与果胶组比较差异均无统计学意义(P>0.05),UC组、ε-聚赖氨酸组、ε-聚赖氨酸+果胶组比较差异均无统计学意义(P>0.05)。(2)对照组结肠组织黏膜上皮结构完整,腺体排列整齐,隐窝正常。UC组黏膜上皮结构不完整,炎症细胞浸润黏膜层2/3以上,以淋巴细胞�
Objective To construct mice models of ulcerative colitis(UC), and to investigate the effects of ε-polylysine, pectin and their mixture on intestinal mucosal barrier in UC mice and its potential mechanism. Methods Forty male BALB/c mice were randomly divided into control group, UC group, ε-polylysine group, pectin group and ε-polylysine + pectin group, with 8 mice each. All mice were pretreated by gavage: control group and UC group were given 20 mL/(kg·d) of drinking water, ε-polylysine group was given 10 mg/(kg·d) of ε-polylysine, pectin group was given 200 mg/(kg·d) of pectin, and ε-polylysine + pectin group was given 10 mg/(kg·d) and 200 mg/(kg·d) pectin. After 4-week gavage, the mice in UC group, ε-polylysine group, pectin group and ε-polylysine + pectin group drank 3% dextroan sulfate solution freely for 9 d to construct UC models, and the mice in control group drank drinking water freely for 9 d. After modeling, the disease activity index(DAI) score was evaluated, the length of the colon was measured, and the pathological morphology of the colon was observed by HE staining method. The serum tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) levels were detected by ELISA. Real-time fluorescence quantitative PCR was used to detect the relative expressions of occludin and zonula occludens-1(ZO-1) mRNAs. Western blot assay was used to detect the relative expressions of Toll-like receptor 4(TLR4), nuclear factor-κB p65(NF-κB p65) and p-NF-κB p65 proteins, and p-NF-κB p65/NF-κB p65 was calculated. Results(1) The DAI score was higher in UC group(6.75±1.91), ε-polylysine group(5.75±1.75), pectin group(3.00±0.76) and ε-polylysine + pectin group(6.00±3.21) than that in control group(0)(P<0.05), was higher in UC group and ε-polylysine + pectin group than that in pectin group(P<0.05), showed no significant difference between ε-polylysine group and pectin group(P>0.05), and among UC group, ε-polylysine group and ε-polylysine + pectin group(P>0.05). The colon length was shorter in UC grou
作者
杨伟明
翟蕾蕾
崔旻
姚萍
YANG Weiming;ZHAI Leilei;CUI Min;YAO Ping(Department of Gastroenterology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang Uygur Autonomous Region 830014,China)
出处
《中华实用诊断与治疗杂志》
2024年第7期656-662,共7页
Journal of Chinese Practical Diagnosis and Therapy
基金
新疆维吾尔自治区自然科学基金(2021D01C334)。
