摘要
优化支架组分和结构以实现内源性成骨是骨组织工程中一项有前景的进展.本研究将蒙脱土作为一种增强相,以插层结构引入到多巴胺介导的仿生有机/无机杂化水凝胶中.通过蒙脱土-聚合物相互作用和多酚介导的多重反应改善了骨再生支架(HDC-HAp-MMT)的理化特性,增强了力学性能和抗降解能力.特别是,持续性释放的有益微量元素为成骨微环境提供了关键的成骨生化信号.该水凝胶在体外促进了骨髓间充质干细胞增殖和成骨分化.同时,在异位成骨模型中,显著增强了矿物沉积(30天后机械增强6.91倍);在原位兔颅骨修复实验中,实现了相对于天然骨80.94%的骨体积分数和85.89%的骨密度.该策略可能代表了一种新型的生物活性骨再生支架的制备方法.
Optimizing scaffold components and architectures to achieve endogenous osteogenesis is a prospective advancement for bone tissue engineering.Here,montmorillonite was introduced as a reinforcing phase into a dopamine-mediated biomimetic organic-inorganic hybrid hydrogel via an intercalation structure,improving the bone regenerative scaffold(HDC-HAp-MMT)architectures through montmorillonite-polymer interactions and polyphenol-mediated multiple reactions,manifested as enhanced mechanics and delayed degradation.Specifically,sustainably releasing beneficial trace elements into the regenerative microenvironment provides crucial osteogenic biochemical signals.Montmorillonite-reinforced hydrogels promoted bone marrow mesenchymal stem cell proliferation and osteogenic differentiation in vitro.The HDC-HAp-MMT scaffolds significantly enhanced mineral deposition(6.91-fold mechanical augmentation after 30 days)in an ectopic osteogenesis model while achieving 80.94%bone volume fractions and 85.89%bone mineral density comparable to natural bone in an orthotopic rabbit cranium repair experiment.This strategy may represent a novel approach for bioactive bone regenerative scaffolds.
作者
童磊
刘泉滢
熊丽
王培磊
赵明达
李星
梁洁
樊渝江
张兴栋
孙勇
Lei Tong;Quanying Liu;Li Xiong;Peilei Wang;Mingda Zhao;Xing Li;Jie Liang;Yujiang Fan;Xingdong Zhang;Yong Sun(National Engineering Research Center for Biomaterials,College of Biomedical Engineering,Sichuan University,Chengdu 610065,China;Sichuan Testing Center for Biomaterials and Medical Devices,Sichuan University,Chengdu 610065,China)
基金
sponsored by National Natural Science Foundation of China(32271419,32071352)。
