摘要
目的探讨高糖高脂诱导的微血管内皮细胞损伤及参松养心胶囊的干预作用。方法2022年6月—2023年2月于络病理论创新转化全国重点实验室开展实验。将人微血管内皮细胞随机分为正常组、模型组和参松养心低、中、高剂量组(0.25、0.5、1.0 mg/L)。除正常组外,其余4组采用高糖高脂建立人微血管内皮细胞损伤模型,并进行相应干预。采用ELISA检测细胞活性,荧光显微镜检测细胞线粒体膜电位,Western-blot检测DRP1、MFN2、GRP78、NF-κB p65、P-selectin、E-selectin、IL-6的蛋白表达水平。结果与正常组比较,模型组的细胞生存活性和线粒体膜电位显著降低,DRP1、GRP78、NF-κB p65、P-selectin、E-selectin、IL-6蛋白表达明显增高,MFN2蛋白表达明显降低(P<0.01或0.05)。与模型组比较,参松养心各剂量组的细胞生存活性和线粒体膜电位均显著升高(P<0.01),GRP78、NF-κB p65、E-selectin的蛋白表达显著降低(P<0.01或<0.05);参松养心低、高剂量组的MFN2蛋白表达显著升高(P<0.05),IL-6蛋白表达显著降低(P<0.05);参松养心中、高剂量组的DRP1、P-selectin蛋白表达显著降低(P<0.05或<0.01)。结论参松养心胶囊可提高微血管内皮细胞的生存活性,维持线粒体稳态,减轻内质网应激,抑制炎性反应,降低黏附分子水平,从而发挥保护高糖高脂诱导损伤的微血管内皮细胞的作用。
Objective To investigate the intervention effect of microvascular endothelial cell injury induced by high glucose and high fat and the intervention effect of Shensong Yangxin Capsule(SSYX).Methods The experiments were conducted from June 2022 to February 2023 at the State Key Laboratory for Innovation and Transformation of Luobing Theory.Human microvascular endothelial cells were randomly divided into the normal group,the model group,the SSYX-L,the SSYX-M and the SSYX-H group(0.25,0.5,1.0 mg/L).In addition to the normal group,the other 4 groups were treated with high glucose and high fat to establish the human microvascular endothelial cell injury model,and carried out corresponding intervention.ELISA was used to detect cell activity,fluorescence microscopy was used to detect cellular mitochondrial membrane potential,and Western blot was used to detect protein levels of DRP1,MFN2,GRP78,NF-κB p65,P-selectin,E-selectin,and IL-6.Results Compared with the normal group,the cell survival activity and mitochondrial membrane potential of the model group were significantly reduced,the protein expressions of DRP1,GRP78,NF-κB p65,P-selectin,E-selectin and IL-6 were significantly increased,and the expression of MFN2 protein was significantly decreased.Compared with the model group,the cell survival activity and mitochondrial membrane potential of each dose group were significantly increased(P<0.01),and the protein expressions of GRP78,NF-κB p65 and E-selectin were significantly decreased(P<0.01 or<0.05).The expression of MFN2 protein in the SSYX-H group was significantly increased(P<0.05),and the expression of IL-6 protein was significantly decreased(P<0.05),and the expression of DRP1 and P-selectin protein in the SSYX-M and SSYX-H groups was significantly decreased(P<0.05 or<0.01).Conclusion Shensong Yangxin Capsule can improve the survival activity of microvascular endothelial cells,maintain mitochondrial homeostasis,reduce endoplasmic reticulum stress,inhibit inflammatory response,and reduce the level of adhesion molec
作者
张洁晗
王同兴
李红蓉
秘红英
张军芳
宋涛
苏敏
Zhang Jiehan;Wang Tongxing;Li Hongrong;Mi Hongying;Zhang Junfang;Song Tao;Su Min(Department of Cardiology Hebei Yiling Hospital,State Key Laboratory for Innovation and Transformation of Luobing Theory,Shijiazhuang 050035,China)
出处
《疑难病杂志》
CAS
2024年第7期866-871,共6页
Chinese Journal of Difficult and Complicated Cases
基金
河北省中医药管理局中医药类科研计划(2022216)。
关键词
高糖高脂
微血管内皮细胞
参松养心胶囊
线粒体
内质网应激
炎性反应
作用机制
High glucose and high fat
Microvascuar endothelial ells
Shensong Yangxin Capsules
Mitochondria
Endoplasmic reticulum stress
Inflammatory reaction
Mechanism of action
