摘要
[目的]筛选犬皮肤源多药耐药菌,同时基于犬防御素设计衍生肽并探究其抗菌作用。[方法]通过K-B药敏纸片法对临床分离的菌株进行筛选,随后以犬β-防御素3(cBD3)的氨基酸序列为模板,采用氨基酸替换的方式对序列进行设计,选取优化肽cBD3-ABU进行化学合成,利用微量肉汤稀释法检测cBD3-ABU对多药耐药菌的抑菌活性。[结果]筛选获得8株犬皮肤源多药耐药菌;通过固相合成法获得纯度达95.61%的cBD3-ABU;cBD3对大肠杆菌、表皮葡萄球菌、伪中间型葡萄球菌和金黄色葡萄球菌的最小抑菌浓度分别为32、64、64、128μg/mL,高浓度cBD3-ABU对犬皮肤源大肠杆菌、表皮葡萄球菌、伪中间型葡萄球菌、金黄色葡萄球菌的抑菌率分别为96.02%、81.85%、87.25%、98.91%。[结论]cBD3-ABU在体外具有良好的抑菌活性,具有进一步开发为治疗性药物的潜力。
[Objectives]The multidrug resistant bacteria from canine skin were screened.The derivative peptide based on canineβ-defensin-3(cBD3)was designed and its antibacterial activity was studied.[Methods]The clinically isolated strains were screened with K-B drug-sensitive disk method.Subsequently,the amino acid sequence ofβ-defensin-3(cBD3)was used as a template to design the sequence with amino acid substitution.The optimized peptide cBD3-ABU was selected for chemical synthesis.The antimicrobial activity of cBD3-ABU against multidrug resistant bacteria was detected with broth microdilution method.[Results]8 strains of multidrug resistant bacteria from canine skin were obtained through screening.CBD3-ABU with a purity of 95.61%was obtained through solid-phase synthesis.The minimum inhibitory concentration(MIC)of cBD3 against Escherichia coli,S.epidermidis,S.pseudintermedius,and S.aureus was 32,64,64,128μg/mL,respectively.The antibacterial rate of high concentration cBD3-ABU against Escherichia coli,Staphylococcus epidermidis,Staphylococcus pseudointermedius and Staphylococcus aureus from canine skin was 96.02%,81.85%,87.25%,and 98.91%,respectively.[Conclusions]CBD3-ABU has good antibacterial activity in vitro and has the potential to be further developed into therapeutic drugs.
作者
吴方溶
欧阳明瑜
赵逸轩
王雨晴
胡长敏
WU Fangrong;OUYANG Mingyu;ZHAO Yixuan;WANG Yuqing;HU Changmin(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)
出处
《养殖与饲料》
2024年第7期20-25,共6页
Animals Breeding and Feed
基金
华中农业大学大学生科技创新基金(2021067)
华中农业大学“名师工作室”培育项目。
关键词
犬β防御素-3
多药耐药菌
最小抑菌浓度
抑菌活性
耐药率
canineβ-defensin-3(cBD3)
multidrug resistant bacteria
minimum inhibitory concentrations(MIC)
antibacterial activity
drug resistance rate
