摘要
目的:探讨人β防御素(human beta-defensin, hBD)-2、3与慢性牙周炎(chronic periodontitis, CP)和高血压之间的关系。方法:前瞻性收集十堰市人民医院口腔科就诊的慢性牙周炎合并高血压患者20例,慢性牙周炎患者20例,牙周健康受试者20例。弗罗里达探针进行牙周指标检测,包括探诊深度(probing depth, PD)、临床附着丧失(clinical attachment level, CAL)以及出血指数(bleeding index, BI)。采用RT-qPCR检测牙龈组织中hBD-2、3 mRNA的表达,免疫组织化学染色检测牙龈组织中hBD-2、3蛋白表达并进行半定量分析。结果:PD、CAL在慢性牙周炎合并高血压组中最高,健康组中最低,差异具有统计学意义(P<0.05)。RT-qPCR测得健康组hBD-2、3 mRNA水平最高,慢性牙周炎组次之,慢性牙周炎合并高血压组最低(P<0.05),且hBD-2、3 mRNA与PD、CAL均呈负相关,hBD-2、3 mRNA与BI呈中等程度负相关。免疫组化结果显示慢性牙周炎组和慢性牙周炎合并高血压组hBD-2、3评分之间差异均不具有统计学意义(P>0.05)。结论:高血压可能是慢性牙周炎患者牙周组织破坏程度加重的危险因素,其原因可能与牙龈组织中hBD-2、3表达降低有关。
Objective:To investigate the relationship between human beta-defensin-2,3 and chronic periodontitis and hypertension.Methods:Twenty patients with chronic periodontitis combined with hypertension(CP+HP group),20 patients with chronic periodontitis and 20 healthy subjects were prospectively collected from the Stomatology department of Shiyan People's Hospital.The Florida probe was used to detect periodontal indicators,including probing depth(PD),clinical loss of attachment(CAL),and bleeding index(BI).RT-qPCR was used to detect the expression of hBD-2 and 3 mRNA in gingival tissue,and immunohistochemical staining was used to detect the expression of hBD-2 and 3 protein in gingival tissue and semi-quantitative analysis was performed.Results:PD and CAL were the highest in chronic periodontitis combined with hy-pertension group and the lowest in healthy group,the difference was statistically significant(P<0.05).The levels of hBD-2 and 3 mRNA were the highest in healthy group,followed by chronic periodontitis,and the lowest in chronic periodontitis com-bined with hypertension group(P<0.05),and hBD-2,hBD-3 mRNA were negatively correlated with PD and CAL,hBD-2,3 mRNA were moderately negatively correlated with BI.Immunohistochemical results showed no significant difference of hBD-2 and hBD-3 scores between chronic periodontitis group and chronic periodontitis combined with hypertension group(P>0.05).Conclusion:Hypertension may be a risk factor for the deterioration of periodontal tissue in patients with chronic peri-odontitis,which may be related to the decreased expression of hBD-2 and 3 in gingival tissue.
作者
周歆
王玉雪
张昊
郑钧元
周永庆
ZHOU Xin;WANG Yu-xue;ZHANG Hao;ZHENG Jun-yuan;ZHOU Yong-qing(Shiyan People's Hospital(People's Hospital Affiliated to Hubei University of Medicine),Hubei Shiyan 442000,China;Department of Stomatology,Shiyan People's Hospital,Hubei Shiyan 442000,China)
出处
《临床口腔医学杂志》
2024年第5期273-277,共5页
Journal of Clinical Stomatology
基金
湖北省卫生健康委员会科研项目(编号:WJ2019F045)。
