摘要
目的:探讨内关、太冲穴位埋线对自发性高血压(SHR)前期大鼠心肌损伤和能量代谢的影响。方法:选择16只4周龄自发性高血压大鼠,随机分为埋线组与模型组(n=8),另取8只同龄的京都种Wistar(WKY)大鼠为对照组。埋线组大鼠定位内关、太冲并进行穴位埋线干预,模型组及对照组仅予以抓取固定干预,实验周期为2周。在干预前1 h及干预2、7、14 d后,测定各组大鼠收缩压(SBP)、舒张压(DBP)、平均动脉压(MAP)及心率(HR);并取心肌组织,采用HE染色比较各组大鼠左心室前壁心肌组织病理改变,电镜观察左心室前壁心肌组织线粒体的结构差异,酶联免疫吸附试验(ELISA)试剂盒测定心肌组织血管紧张素Ⅱ(AngⅡ)水平,三磷酸腺苷(ATP)检测试剂盒测定心肌组织中ATP水平。结果:干预2、7和14 d后,与模型组比较,埋线组大鼠DBP和MBP显著降低(P<0.01);干预7 d和14 d后,与模型组比较,埋线组大鼠SBP明显降低(P<0.01)。3组大鼠HR比较,差异无统计学意义(P>0.05)。模型组心肌细胞排列紊乱,心肌细胞横径和细胞间隙明显增大;与模型组比较,埋线组心肌细胞排列规则,心肌细胞横径明显减小(P<0.05),细胞间隙基本正常。在高倍透射电镜下,模型组线粒体排列紊乱,形状不规则,线粒体密度减小,埋线组较模型组有所改善。与模型组比较,埋线组左心室心肌组织中ATP水平升高,AngⅡ水平降低(P<0.05)。结论:内关、太冲穴位埋线可调节自发性高血压前期大鼠心肌能量代谢,对改善心肌损伤和降低血压水平有积极作用。
Objective:To explore the effect of acupoint catgut embedding at Neiguan(PC 6)and Taichong(LR 3)on myocardial injury and energy metabolism in spontaneously prehypertensive rats.Methods:Totally 164-week-old spontaneously hypertensive(SHR)rats were randomly divided into model group and acupoint catgut embedding group,and 8 Kyoto Wistar(WKY)rats of the same age were selected as the control group.The rats in acupoint catgut embedding group were located at the Neiguan(PC 6)and Taichong(LR 3)acupoints and were underwent acupoint catgut embedding intervention.The rats were treated with grasping fixation intervention in model group and control group.The trial period was 2 weeks.The systolic blood pressure(SBP),diastolic blood pressure(DBP),mean arterial pressure(MAP)and heart rate(HR)were measured,one hour before the intervention and 2,7,and 14 days after the intervention.HE staining was used to observe the pathological changes of myocardial tissue in the anterior wall of the left ventricle of all groups.The structural differences of mitochondria in myocardial tissue of the left ventricular anterior wall of rats were observed under electron microscope.The myocardial levels of angiotensinⅡ(AngⅡ)were determined by ELISA kits,and the myocardial level of ATP was measured with an ATP kits.Results:Compared with model group,the DBP and MBP were significantly reduced in acupoint catgut embedding group,2,7 and 14 days after the intervention(P<0.01),and the SBP decreased in acupoint catgut embedding group,7 and 14 days after the acupoint embedding(P<0.01).There was no statistically significant difference in HR among 3 groups(P>0.05).In terms of histopathological staining,myocardial cells in model group were in disorder,and the cell volume and gap were significantly increased with inflammatory cell infiltration.Compared with model group,the arrangement of myocardial cells was regular and the transverse diameter of myocardial cells was significantly reduced in acupoint catgut embedding group(P<0.05),with basically normal intercel
作者
黄国华
李湘海
陈小青
HUANG Guohua;LI Xianghai;CHEN Xiaoqing(Guizhou Medical University,Guiyang Guizhou 550025,China)
出处
《中医药导报》
2024年第6期34-38,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2020-053)。
关键词
自发性高血压
高血压前期
内关穴
太冲穴
穴位埋线
心肌损伤
心肌能量代谢
大鼠
spontaneous prehypertensive
prehypertensive stage
Neiguan(PC 6)
Taichong(LR 3)
acupoint embedding
myocardial injury
myocardial energy metabolism
rat
