摘要
目的:观察针刺对自发性高血压大鼠(SHR)血压、粪便短链脂肪酸(SCFAs)及toll样受体4(TLR4)/髓样分化因子88(My D88)/核因子κB(NF-κB)信号通路的影响,探讨针刺降压的作用机制。方法:将24只SPF级雄性SHR随机分为模型组、西药组、针刺组和假针组,每组6只;另设6只Wistar-Kyoto(WKY)大鼠为空白组。西药组予氢氯噻嗪溶液灌胃;针刺组针刺双侧“人迎”“足三里”,每次20 min;假针组针刺双侧“人迎”“足三里”附近的非经非穴点,每次20 min。均每日1次,持续4周。干预前及干预第1、2、3、4周后测量各组大鼠尾动脉收缩压(SBP);HE染色观察大鼠结肠组织形态;气相色谱-质谱联用仪检测大鼠粪便SCFAs含量;ELISA法检测大鼠血清白细胞介素(IL)-6、IL-1β和肿瘤坏死因子-α(TNF-α)含量;Western Blot法检测大鼠肠系膜动脉中TLR4、My D88和NF-κB p65蛋白表达。结果:与空白组比较,模型组大鼠SBP升高(P<0.05),结肠组织有明显病理变化,粪便SCFAs含量降低(P<0.05),血清IL-1β、IL-6和TNF-α含量升高(P<0.05),肠系膜动脉中TLR4、My D88、NF-κB p65蛋白表达升高(P<0.05)。与模型组比较,针刺组和西药组大鼠干预2、3、4周后SBP降低(P<0.05),血清IL-1β、IL-6和TNF-α含量降低(P<0.05);针刺组大鼠结肠组织黏膜上皮完整,肠腺数量丰富,粪便SCFAs含量升高(P<0.05),肠系膜动脉中TLR4、My D88、NF-κBp65蛋白表达降低(P<0.05)。与假针组比较,针刺组干预2、3、4周后SBP降低(P<0.05),粪便SCFAs含量升高(P<0.05),血清IL-1β、IL-6和TNF-α含量降低(P<0.05),肠系膜动脉中TLR4、My D88、NF-κB p65蛋白表达降低(P<0.05)。结论:针刺双侧“人迎”“足三里”可有效降低SHR血压,其机制可能与调节粪便SCFAs含量、抑制TLR4/My D88/NF-κB信号通路有关。
Objective To observe the effects of acupuncture on blood pressure,fecal short-chain fatty acids(SCFAs)and toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear factor-κB(NF-κB)signaling pathway in spontaneously hypertensive rats(SHR),and to explore the mechanism of acupuncture for anti-hypertension.Methods Twenty-four male SHR of SPF grade were randomly divided into a model group,a western medication group,an acupuncture group and a sham acupuncture group,with 6 rats in each group,and 6 male Wistar-Kyoto rats were selected as the blank group additionally.Hydrochlorothiazide solution was given by gavage in the western medication group;acupuncture was applied at bilateral"Renying"(ST 9)and"Zusanli"(ST 36)in the acupuncture group,20 min a time;acupuncture was applied at the non-meridian and non-acupoint points close to bilateral"Renying"(ST 9)and"Zusanli"(ST 36)in the sham acupuncture group,20 min a time.The intervention was adopted once a day for 4 weeks continuously in each group.The systolic blood pressure(SBP)of the caudal artery was measured before intervention and after 1,2,3 and 4 weeks of intervention.After intervention,the morphology of colonic tissue was observed by HE staining;the fecal level of SCFAs was detected by gas chromatography;the serum levels of interleukin(IL)-6,IL-1β and tumor necrosis factor-α(TNF-α)were detected by ELISA;the protein expression of TLR4,My D88 and NF-κB p65 in the mesenteric artery was detected by Western blot.Results Compared with the blank group,in the model group,the SBP was increased(P<0.05),significant pathological changes could be found in the colonic tissue,the fecal SCFAs level was decreased(P<0.05),the serum levels of IL-6,IL-1β and TNF-α were increased(P<0.05),the protein expression of TLR4,My D88 and NF-κB p65 in the mesenteric artery was increased(P<0.05).Compared with the model group,the SBP after 2,3 and 4 weeks of intervention was decreased(P<0.05),the serum levels of IL-6,IL-1β and TNF-α were decreased(P<0.05)in the acupuncture
作者
丁志敏
陈婷玉
张丽红
金小琴
刘斌
冯晓东
郑婕
DING Zhimin;CHEN Tingyu;ZHANG Lihong;JIN Xiaoqin;LIU Bin;FENG Xiaodong;ZHENG Jie(School of Rehabilitation Medicine,Henan University of CM,Zhengzhou 450046,China;Rehabilitation Center,First Affiliated Hospital of Henan University of CM)
出处
《中国针灸》
CAS
CSCD
北大核心
2024年第6期661-668,共8页
Chinese Acupuncture & Moxibustion
基金
河南省高等学校重点科研项目:21A360013
河南省自然科学基金项目:202300410260
河南中医药大学研究生科研创新项目:2021KYCX031
河南省“双一流”创建学科中医学科学研究专项项目:HSRP-DFCTCM-T-9。
关键词
高血压
针刺
人迎
足三里
短链脂肪酸
炎性反应
TLR4/MyD88/NF-κB信号通路
hypertension
acupuncture
Point ST 9(Renying)
Point ST 36(Zusanli)
short-chain fatty acids(SCFAs)
inflammatory reaction
TLR4/MyD88/NF-κB signaling pathway
