摘要
[目的]探究1-脱氧-D-木酮糖-5-磷酸还原异构酶(DXR)基因在紫丁香单萜类物质合成中的作用,为紫丁香花香分子育种提供有效的基因资源和理论基础。[方法]以紫丁香花瓣为材料,克隆了SoDXR基因,并对其进行生物信息学和亚细胞定位分析、不同花发育时期和器官组织中的qRT-PCR表达分析以及探究其在金鱼草花瓣中异源瞬时过表达后的功能。[结果]SoDXR基因全长为1425 bp,编码474个氨基酸,编码的蛋白具有保守的DXP_reductoisom结构域,与桂花和油橄榄的相似性高达95%。亚细胞定位显示该蛋白主要定位于质体,与软件预测结果一致。伴随着开花,SoDXR表达水平呈先升高后降低的趋势,在初开期最高,其在各个器官组织中均有表达,花瓣中表达量最高,茎中表达量最低。瞬时过表达表明,SoDXR基因的表达水平显著高于对照,促进了主要单萜成分罗勒烯和月桂烯的释放,释放量分别增加了15.03倍和4.83倍。[结论]SoDXR基因正调控紫丁香单萜类物质的合成,表明DXR基因在花香次级代谢物合成中起到重要作用。
[Objective]The purpose of this study is to examine the role of the DXR gene,encoding 1-deoxy-D-xylulose-5-phosphate reductoisomerase,in monoterpenoids synthesis in lilac(Syringa oblata),and to provide genetic resources and theoretical basis for the molecular breeding of floral fragrance of lilac.[Methods]Firstly,the SoDXR gene was cloned.Then,bioinformatics and subcellular localization analysis were performed,and qRT-PCR was used to analyze the gene expression patterns in different floral development stages and tissues.Finally,transient overexpression of SoDXR in the petals of snapdragon(Antirrhinum majus)was detected.[Results]ORF of SoDXR was 1425 bp,encoding 474 amino acids.SoDXR protein contained the conserved domain of DXP_reductoisom,which had a similarity of up to 95%with Osmanthus fragrans and Olea europaea.Subcellular localization analysis showed that the protein was mainly located in the plastids,consistent with the software prediction.With flowering,the expression level of SoDXR was increased and then decreased,with the highest expression in the initial opening stage.SoDXR was differentially expressed in various tissues with the highest in petals and the lowest in stems.Transient overexpression revealed that the expression of SoDXR was higher than the control.Moreover,the main floral volatile components in snapdragon petals,myrcene and ocimene,were increased by 15.03 and 4.83 times,respectively.[Conclusion]The SoDXR gene positively regulates monoterpenoids synthesis in lilac,and may play an important role in the synthesis of secondary metabolites of floral fragrance.
作者
杨艺慧
邹吉睿
马波
冷平生
胡增辉
吴静
YANG Yihui;ZOU Jirui;MA Bo;LENG Pingsheng;HU Zenghui;WU Jing(College of Landscape Architecture,Beijing University of Agriculture,Beijing 102206,China;Beijing Laboratory of Urban and Rural Ecological Environment,Beijing 102206,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2024年第6期912-919,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
北京市教委生态修复工程学高精尖学科建设项目。
