摘要
木葡聚糖内转糖苷酶/水解酶(XTH)属于糖苷水解酶16家族(GH16),是一类介导木葡聚糖(XyG)-纤维素骨架构建和重组的酶。为探明XTH家族基因在金鱼草(Antirrhinum majus)中的潜在生物学功能,通过生物信息学分析,结合转录组测序(RNA-seq)和实时荧光定量聚合酶链式反应(qRT-PCR)探究了XTH家族基因分别在金鱼草瓣化和非瓣化雄蕊以及抗感核盘菌材料中的表达水平。结果表明,鉴定出的33个AmXTH蛋白主要保守基序为ExDxE,分为3个亚组。AmXTH基因启动子的顺式作用元件多为生长发育、抗病及抗逆类。经RNA-seq和qRT-PCR验证,最终挖掘出4个正向介导抗病的关键候选基因(AmXTH3、14、18和33), 1个负向介导抗病的关键候选基因(AmXTH23), 12个正向介导雄蕊瓣化的关键候选基因(AmXTH1、7、9、11、21、22、23、24、26、28、29和33)以及2个负向介导雄蕊瓣化的关键候选基因(AmXTH15和31);其中AmXTH23和AmXTH33可能同时在金鱼草抗核盘菌和雄蕊瓣化中发挥作用。该研究初步挖掘出参与金鱼草抗核盘菌及雄蕊瓣化的AmXTH候选基因,为进一步揭示其生物学功能奠定了基础。
Xyloglucan endotransglucosylase/hydrolases(XTH)belongs to glycoside hydrolase,family 16(GH16)and it is a class of enzymes that mediate the construction and recombination of xyglucan-cellulose skeleton.To explore the potential biological functions of XTH family genes in Antirrhinum majus.In this paper,bioinformatics analysis,RNA-seq analysis and qRT-PCR were used to investigate the expression levels of the family genes in the petalized and non-petalized stamens and disease-resistant materials.The results showed that the main conserved motif from 33 identified AmXTH proteins was ExDxE,which could be divided into 3 subgroups.Most of the cis-acting elements of AmXTH promoter are growth and development,disease resistance and stress resistance.RNA-seq and qRT-PCR revalidation finally unearthed four positive candidate genes(AmXTH3,14,18,and 33)and one candidate genes(AmXTH23)for nagatively mediated Sclerotinia sclerotiorum resistance.There were 12 positive AmXTH candidate genes(AmXTH1,7,9,11,21,22,23,24,26,28,29 and 33)and 2 negative AmXTH candidate genes(AmXTH15 and 31).Among them,AmXTH23 and 33 may play a role in both of the A.majus resistant to S.sclerotiorum and stamen petalization.In this study,the candidate AmXTH genes involved in S.sclerotiorum resistance and stamen petalization of A.majus were preliminarily excavated,which laid a foundation for further revealing the gene function.
作者
赵晗茜
宋佳怡
杨洁
赵永晶
夏文念
顾伟卓
汪仲毅
杨楠
胡慧贞
Hanqian Zhao;Jiayi Song;Jie Yang;Yongjing Zhao;Wennian Xia;Weizhuo Gu Zhongyi Wang;Nan Yang;Huizhen Hu(Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization,College of Landscape Architecture and Horticulture Sciences,Southwest Forestry University,Kunming 650224,China)
出处
《植物学报》
CAS
CSCD
北大核心
2024年第2期188-203,共16页
Chinese Bulletin of Botany
基金
国家自然科学基金地区科学基金(No.32360070)
国家自然科学基金青年基金(No.31901571)
西南林业大学科研启动项目(No.01102-112109)
云南省农业基础联合面上项目(No.202301BD070001-072)。
关键词
金鱼草
木葡聚糖内转糖苷酶/水解酶家族
核盘菌抗性
雄蕊瓣化
基因差异表达
Antirrhinum majus
xyloglucan endotransglucosylase/hydrolases family
Sclerotinia sclerotiorum resistance
stamen petalization
differential gene expression
