摘要
目的优化鸡根标准汤剂提取工艺,并建立其质量标准。方法在单因素试验基础上,以浸泡时间、加水量、一煎时间为影响因素,3,6′-二芥子酰基蔗糖含量、出膏率的综合评分为评价指标,响应面法优化提取工艺。测定3,6′-二芥子酰基蔗糖含量及转移率,建立HPLC特征图谱,进行聚类分析、主成分分析和正交偏最小二乘判别分析。结果最佳条件为浸泡时间60 min,加水量(12+11)倍,煎煮时间(47+20)min,综合评分为97.98分。15批标准汤剂平均出膏率为14.182%,转移率为20.468%;其特征图谱中有6个共有峰,相似度均大于0.9(除S4、S8外)。各批标准汤剂聚为2类,3种主成分累积方差贡献率达91.4%,峰2、6为质量标志物。结论该方法精密稳定,重复性好,可用于鸡根标准汤剂的制备及质量控制。
AIM To optimize the extraction process for Jigen Standard Decoction,and to establish its quality standard.METHODS With soaking time,water addition and first decoction time as influencing factors,comprehensive score for 3,6′-disinapoyl sucrose content and yield rate as an evaluation index,the extraction process was optimized by response surface method on the basis of single factor test.The content and transfer rate of 3,6′-dimustayl sucrose were determined,after which HPLC characteristic chromatograms were established,cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were performed.RESULTS The optimal conditions were determined to be 60 min for soaking time,(12+11)times for water addition,and(47+20)min for decoction time,the comprehensive score was 97.98.Fifteen batches of standard decoctions demonstrated the average yield rate and transfer rate of 14.182% and 20.468%,respectively,whose characteristic chromatograms existed six common peaks with the similarities of more than 0.9(except for S4,S8).Various batches of standard decoctions were clustered into two types,three principal components displayed the acumulative variance contribution rate of 91.4%,peaks 2,6 were quality markers.CONCLUSION This precise,stable and reproducible method can be used for the preparation and quality control of Jigen Standard Decoction.
作者
杨国春
杨亚芳
徐素娥
柯瑾
陈凌云
侯安国
金文彬
YANG Guo-chun;YANG Ya-fang;XU Su-e;KE Jin;CHEN Ling-yun;HOU An-guo;JIN Wen-bin(College of Chinese Materia Medica,Yunnan University of Chinese Medicine,Kunming 650500,China;Yunnan Provincial Key Laboratory for Researches on External Drug Delivery System and Preparation Technology in Colleges and Universities,Kunming 650500,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第6期1773-1781,共9页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(82104067)
云南省重大科技专项(202102AA310027)
云南省中医药学分子生物学重点实验室项目(2019DG016)
云南省基础研究专项(202001AU070133)
云南省中医联合专项(202001AZ070001-091)。
关键词
鸡根标准汤剂
提取工艺
质量标准
响应面法
HPLC特征图谱
聚类分析
主成分分析
正交偏最小二乘判别分析
Jigen Standard Decoction
extraction process
quality standard
response surface method
HPLC characteristic chromatograms
cluster analysis
principal component analysis
orthogonal partial least squares discriminant analysis
