摘要
采用浸提和柱层析等手段从金丝楸心材中制备抗菌组合物,该组合物体外抗金黄色葡萄球菌(S.aureus ATCC 25923)最小抑菌浓度(MIC)值为64.0μg/mL。运用HPLC技术,结合标准品比对法,鉴定该组合物主要化学成分为梓木内酯和4,9-二羟基-α-拉帕醌,相对含量,分别为62%和23%。通过添加一定量的金丝楸抗菌组合物,制得金丝楸抑菌皂,并评价了药物浓度与抑菌活性的相关性。当添加浓度为90μg/mL时,制得的抑菌皂与市售三种抑菌皂抑菌效果一致。
The antibacterial composition was prepared from the heartwood of catapla bungei“Jinsi”by means of extraction and column chromatography.The minimum inhibitory concentration(MIC)of the combination against S.aureus ATCC 25923 in vitro was 64.0μg/mL.The main chemical components of the composition were identified as catalpalactone and 4,9-dihydroxy-α-lapachone by HPLC technology and standard comparison method,relative content,62%and 23%respectively.The antibacterial soap of catapla bungei“Jinsi”was prepared by adding a certain amount of antibacterial composition of catapla bungei“Jinsi”,and the correlation between the drug concentration and the antibacterial activity was evaluated.When the added concentration is 90μg/mL,the antibacterial effect of the prepared antibacterial soap is consistent with that of the three commercially available antibacterial soaps.
作者
吴晨婷
陈贤飞
薛莲
李君桐
陈晖
金卫华
尚文娟
WU Chen-ting;CHEN Xian-fei;XUE Lian;LI Jun-tong;CHEN Hui;JIN Wei-hua;SHANG Wen-juan(Key Laboratory of Functional Yeast,China National Light Industry,College of Biological and Pharmaceutical Sciences,China Three Gorges University,Hubei Yichang 443002;HEC API Yichang Co.,Ltd.,HEC 3rd Industrial Park,Hubei Yichang 443311;Three Gorges University Medical College,China Three Gorges University,Hubei Yichang 443002;Center for Industrial Microbiology and Brewing Technology,Angel Yeast Co.,Ltd.,Hubei Yichang 443003;Medical College,Hubei Three Gorges Polytechnic,Hubei Yichang 443000,China)
出处
《广州化工》
CAS
2024年第4期92-95,共4页
GuangZhou Chemical Industry
基金
宜昌市自然科学研究项目(金丝楸心材化学成分及其抗肿瘤活性研究)。
关键词
金丝楸
抑菌皂
抑菌
catapla bungei“Jinsi”
antibacterial soap
bacteriostatic
